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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Does not meet important criteria of today standard methods. Study probably adequately conducted at the time (1956), however, only limited experimental details; not equivalent to modern guidelines.
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Toxicological studies of certain alkylated benzenes and benzene.
Author:
Wolf, M.A. et al.
Year:
1956
Bibliographic source:
AMA Arch Ind Health 14: 387-398
Reference Type:
secondary source
Title:
European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.
Author:
European Union
Year:
2007
Bibliographic source:
European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.

Materials and methods

Principles of method if other than guideline:
Two drops of undiluted liquid styrene were applied to the right eye of an unspecified number of albino rabbits. Visual inspection for irritation and corneal injury (both internal and external) was made on the treated eyes at 3 minutes, 1 hour, and 1, 2 and 7 days post-instillation.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Styrene
EC Number:
202-851-5
EC Name:
Styrene
Cas Number:
100-42-5
Molecular formula:
C8H8
IUPAC Name:
ethenylbenzene
Details on test material:
- Name of test material (as cited in study report): Styrene
- Physical state: liquid
- Analytical purity: > 99%

Test animals / tissue source

Species:
rabbit
Strain:
other: white rabbit
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: Biochemical Research Department, Dow Chemical Company from a heterogenous stock
- Diet: commercial diet supplemented by alfalfa hay

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
two drops
Duration of treatment / exposure:
single application of two drops
Observation period (in vivo):
3 minutes, 1 hour, and 1, 2 and 7 days post-instillation
Number of animals or in vitro replicates:
no data

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
conjunctivae score
Time point:
other: 3 min, 1 h, and 1, 2 and 7 days
Score:
>= 0
Reversibility:
not specified
Irritation parameter:
cornea opacity score
Time point:
other: 3 min, 1 h, and 1, 2 and 7 days
Score:
>= 0
Reversibility:
not specified

Any other information on results incl. tables

Moderate conjunctival irritation (inflammation and slight swelling of the eyelids) and slight, transient corneal injury (perceptible superficial necrosis involving <50% of the lens) were reported.

Applicant's summary and conclusion

Interpretation of results:
other: not interpretable