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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Does not meet important criteria of today standard methods. Study probably adequately conducted at the time (1956), however, only limited experimental details; not equivalent to modern guidelines.
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Toxicological studies of certain alkylated benzenes and benzene.
Author:
Wolf, M.A. et al.
Year:
1956
Bibliographic source:
AMA Arch Ind Health 14: 387-398
Reference Type:
secondary source
Title:
European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.
Author:
European Union
Year:
2007
Bibliographic source:
European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.

Materials and methods

Principles of method if other than guideline:
Repeated occlusive skin application of 10-20 doses of undiluted styrene over 2-4 weeks to the ear and abdomen.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Styrene
EC Number:
202-851-5
EC Name:
Styrene
Cas Number:
100-42-5
Molecular formula:
C8H8
IUPAC Name:
ethenylbenzene
Details on test material:
- Name of test material (as cited in study report): Styrene
- Analytical purity: > 99%

Test animals

Species:
rabbit
Strain:
other: white rabbit
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Biochemical Research Department, Dow Chemical Company from a heterogenous stock
- Diet: commercial diet supplemented by alfalfa hay

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
no data
Duration of treatment / exposure:
10 to 20 applications were made to the ear and onto the shaved abdomen over a period of two to four weeks
Observation period:
2-4 weeks, observation daily
Number of animals:
no data
Details on study design:
Animals were observed daily and were weighed weekly.

Results and discussion

In vivo

Results
Irritation parameter:
other: qualitative observation of erythema and necrosis
Time point:
other: after 2-4 weeks
Score:
> 0 - < 4

Any other information on results incl. tables

Moderate “definite erythema” with “slight necrosis” (development of a thin layer of devitalised tissue which resulted in exfoliation) on the surface skin. No other details are available. Numerical score not assignable.

Applicant's summary and conclusion

Interpretation of results:
other: not interpretable