Crotonaldehyde

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: gene mutation

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: publication

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 477 (Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster)

Principles of method if other than guideline:

Dose administered by feeding

GLP compliance:

not specified

Type of assay:

Drosophila SLRL assay

Test material

Test material information

Constituent 1

Reference substance name:

Crotonaldehyde

EC Number:

224-030-0

EC Name:

Crotonaldehyde

Cas Number:

4170-30-3

Molecular formula:

C4H6O

IUPAC Name:

but-2-enal

Details on test material:

molecular weight: 70.10
Source: Aldrich (JD 031397)
purity: 98-99%

Test animals

Species:

Drosophila melanogaster

Strain:

other: Canton-S

Sex:

male

Details on test animals or test system and environmental conditions:

standard temperature: 23-25C

Administration / exposure

Route of administration:

oral: feed

Vehicle:

Solvent of choice for adult feeding study was solution of 5% sucrose in distilled water or 5% sucrose buffered to pH 6.8 with phosphate buffer

Details on exposure:

adult feeding : Males were treated for 3 days in glass shell vials containing a glass fiber disc soaked with 0.2 - 0.5 ml of solution

Duration of treatment / exposure:

feeding: 3 days

Frequency of treatment:

NA

Post exposure period:

for feeding: none

Doses / concentrations

Remarks:

Doses / Concentrations:
4000 ppm
Basis:
nominal conc.

No. of animals per sex per dose:

unknown

Positive control(s):

unknown

Examinations

Tissues and cell types examined:

not applicable

Details of tissue and slide preparation:

Not applicable

Evaluation criteria:

Lethal mutations were declared if no wild-type males were recovered among 20 or more Basc males or Bascl +/_ females. Retests were performed to confirm lethality. In these cases where a few F2 or F3 wild-type males survived among a large number of progeny, a lethal was declared if wild type males were less than 5% of the total Basc males or BACSL +/_ females.

A translocation was judged to have occured only in crosses with total counts of 20 or more progeny. Retests were performed to confirm translocations. T (Y;2;3) events were counted as two translocations.

Statistics:

Lethal test: normal test [Margolin et al 1983]. Calculation of lethal frequencies and statistical tests were performed after clusters were removed.

Reciprocal translocation test: Conditional binomial test [Kastenbaum and bowman, 1970]

cluster analysis: by use of the formula for the cumulative Poisson distibution [Owens et al 1962] with a 0.01 alpha value. All data from a parental male producing a cluser were excluded.

Results and discussion

Additional information on results:

Negative for mutations if crotonaldehyde was administered via food

Any other information on results incl. tables

Results of Sex Linked Recessive Lethal Mutation Tests

Dose	Route of Administration	Percent Mortality	Percent sterility	Lethals			Tests			Total lethals	Total tests	Percent lethals
				Br1	Br2	Br3	Br1	Br2	Br3
4000	Feeding	3	0	2	1	3	3150	2821	2606	6	8577	0.07
0	Feeding			1	2	1	2399	2284	2107	4	6790	0.06

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative oral administration
Crotonaldehyde was negative in vivo for inducing mutation upon oral administration.

Executive summary:

Crotonaldehyde was tested for mutagenicity in Drosphilia melanogaster by adult feeding for the induction of sex-linked recessive lethal mutations in meotic and postmeitic germ cells stages on Canton-S males. Crotonaldehyde adminsitered via oral feed was not mutagenic in this assay.