Crotonaldehyde

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Acceptable (pre-GLP; pre-guidelines), study containing basic data which suggests that basic scientific principles have been met. This is sufficient to judge the results reliable as a contribution to the understanding of the toxicity of this substance.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1950

Materials and methods

Principles of method if other than guideline:

other

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Crotonaldehyde

EC Number:

224-030-0

EC Name:

Crotonaldehyde

Cas Number:

4170-30-3

Molecular formula:

C4H6O

IUPAC Name:

but-2-enal

Test animals

Species:

other: rat and mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

Subcutaneous injections of aqueous solutions of crotonaldehyde at 10% concentration were applied to the rats and 1% to the mice. Animals were observed for 21 days.

Administration / exposure

Route of administration:

subcutaneous

Vehicle:

water

Details on exposure:

A single exposure was applied.

Doses:

0.1 - 0.18 g/kg for the rats
0.12 - 0.26 g/kg for the mice

No. of animals per sex per dose:

96 animals total

Control animals:

no

Details on study design:

no additional data

Statistics:

no data

Results and discussion

Effect levels

Dose descriptor:

LD50

Effect level:

140 mg/kg bw

Mortality:

There was a total mortality of 43 animals, 6 between 0-1hr, 26 1and 6 hr and 11 between 6-24 hr.

Clinical signs:

intense excitation, nose, ears and feet became strongly red.

Gross pathology:

Lungs: hyperemia, haemorrhages, perivascular edema, slight peribronchrial pneumonic changes.
Heart, liver, kidneys: hyperemia
Other organs: no changes

Other findings:

Survivors appeared recovered after 24 to 48 hr.

Any other information on results incl. tables

"A few minutes after injection a stage of intense excitation activity set in, lasting 10 -15 minutes, during which the animals ran about squeaking in the cage. At the same time peripheral parts of the body (nose, ears and feet) became strongly reddened. With the larger doses there were also general tremors or convulsions. The peripheral reddening subsided and disappeared in about an hour. With the larger doses death occured during or close to the stage of excitation. With the smaller doses the animals died most oftern during the night after the injections and no death occured later than 24 hours afterwards. The survivors seemed to have recovered after one to two days."

Applicant's summary and conclusion

Conclusions:

Crotonaldehyde resulted toxic via subcutaneous and more toxic in rats than in mice.

Executive summary:

A subcutaneous toxicity study with crotonaldehyde indicated that the substance is toxic to rats and mice and suggested that the lungs and respiratory tract were the target organs. The LD50 was 0.16 g/kg in the mouse and 0.14 g/kg in the rat.