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Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from a secondary source.

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Toxicity to microorganism study was carried out for time period of 5 hrs.

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

- Concentrations: (0, 500, 1000, 1500 and 2000 ul/l),
- Sampling method: Two were treated with HgCl2 solution to kill the bacteria, and serve to determine auto-oxidation grades of the test substance. Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content. At the end of testing HCl was added to stop biochemical processes.

Vehicle:

no

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

5 h

Test temperature:

25 °C

Nominal and measured concentrations:

(0, 500, 1000, 1500 and 2000 ul/l),

Details on test conditions:

TEST SYSTEM
- Test vessel: Four 100-ml von Loh bottles
- Type (delete if not applicable): closed, glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations were sealed without air.
- Aeration: No
- Control vessel details : Two were treated with HgCl2 solution to kill the bacteria, and serve to determine auto-oxidation grades of the test substance. Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content.

Reference substance (positive control):

not specified

Duration:

5 h

Dose descriptor:

EC10

Effect conc.:

1 520 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Other details not known

Duration:

5 h

Dose descriptor:

EC50

Effect conc.:

> 1 520 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Other details not known

Validity criteria fulfilled:

not specified

Conclusions:

Based on effect on growth inhibition of test bacteria, the 5 hrs EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l.

Executive summary:

Toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.

Reference 2

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from a secondary source.

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Toxicity to microorganism study was carried out for time period of 16 hrs.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: Bacteria

Test type:

static

Water media type:

freshwater

Total exposure duration:

16 h

Reference substance (positive control):

not specified

Duration:

16 h

Dose descriptor:

IC50

Effect conc.:

435 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Other details not known

Validity criteria fulfilled:

not specified

Conclusions:

Based on growth inhibition of bacteria, the 16 hrs IC50 value was determined to be 435 mg/l.

Executive summary:

Toxicity to microorganism study was carried ot for time period of 16 hrs. Bacteria was used as test innoculum. Based on growth inhibition of bacteria, IC50 value was determined to be 435 mg/l. Based on IC50 value test chemical was considered as non-toxic to microorganism.

Reference 3

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data for the target chemical is summarized based on the read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Toxicity to microorganism study was carried out for time period of 16 hrs.

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

WoE-3 : - Concentrations: (0, 500, 1000, 1500 and 2000 ul/l),
- Sampling method: Two were treated with HgCl2 solution to kill the bacteria, and serve to determine auto-oxidation grades of the test substance. Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content. At the end of testing HCl was added to stop biochemical processes.

Vehicle:

no

Test organisms (species):

other: WoE-2 : Other bacteria WoE-3 : Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test temperature:

25 °C

Nominal and measured concentrations:

WoE-3 : (0, 500, 1000, 1500 and 2000 ul/l),

Details on test conditions:

WoE-3 :
TEST SYSTEM
- Test vessel: Four 100-ml von Loh bottles
- Type (delete if not applicable): closed
- Aeration: No

Reference substance (positive control):

yes

Remarks:

WoE-3 :Nine control bottles without the test substance were used as reference; four of these contained HgCl2 to determine the final oxygen content

Duration:

16 h

Dose descriptor:

IC50

Effect conc.:

435 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Other details not known

Duration:

5 h

Dose descriptor:

EC10

Effect conc.:

1 520 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Other details not known

Validity criteria fulfilled:

not specified

Conclusions:

Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.

Executive summary:

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on microorganism. The studies are as mentioned below:

 

First study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.

 

Second study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.

 

Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.

 

Based on above information test chemical was considered as non-toxic to microorganism.

Description of key information

Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.

Key value for chemical safety assessment

EC50 for microorganisms:

1 520 mg/L

Additional information

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on microorganism. The studies are as mentioned below:

 

First study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.

 

Second study includes toxicity to microorganism study was carried out for time period of 5 hrs. Study was performed under static condition. Pseudomonas putida was used as test bacteria. Tes was carried out at temperature 25°C. Four 100-ml von Loh bottles with ground glass stoppers were coated with the culture solution, the bacterial suspension, and the test substance in staged concentrations (0, 500, 1000, 1500 and 2000 ul/l), were sealed without air, and were incubated for 5 hours. Two were treated with HgCl2 solution to kill the bacteria. Nine control bottles without the test substance were used as reference, four of these contained HgCl2 to determine the final oxygen content. Based on effect on growth inhibition of test bacteria, EC10 value was determined to be 1520 mg/l, hence EC50 value was considered as > 1520 mg/l. Thus, based on EC50 alue test chemical was considered as non-toxic to test bacteria.

 

Based on effect on growth inhibition of test bacteria, the 16 hrs EC50 values were determined to be in range of 435 to1520 mg/l respectively.

 

Based on above information test chemical was considered as non-toxic to microorganism.