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Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from a secondary source.

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EEC, C.3

Principles of method if other than guideline:

Toxicity to algae study was carried out for time period of 72 hrs.

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

- Concentrations: concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l
- Sampling method: 1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test temperature:

24 +/- 2 deg C

pH:

6.9-7.6 (beginning of test); 8.3-8.9 (end of test)

Nominal and measured concentrations:

concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Aeration: Yes

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: Yes
- Light intensity and quality: 8000 lux, white

Reference substance (positive control):

not specified

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

190 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Other details not known

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 420 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Other details not known

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

53 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Other details not known

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

91 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Other details not known

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

255 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Other details not known

Validity criteria fulfilled:

not specified

Conclusions:

Based on effect of biomass of test algae, the 72 hrs NOEC, EC10 and EC50 values were determined to be 53, 91 and 255 mg/l respectively. And based on growth rate EC10 an EC50 values were 190 and > 420 mg/l.

Executive summary:

Toxicity to algae study was carried out for time period of 72 hrs by following the method of Directive 92/69/EEC, C.3. Study was performed under static condition at temperature 24 +/- 2 deg C. Scenedesmus subspicatus was used as test organism. Test medium used was as described in guideline 92/69/EEC C.3. (1992). Stock solution was prepared as1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered. Green algae were exposed at concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l. Light intensity used was 8000 lux, white. pH at beginning of test 6.9-7.6 and end of test was 8.3-8.9 respectively. sterile-aerated Erlenmeyer flasks was used as test vessel. The analyses were carried out by measurement of dissolved organic carbon. The DOC content was determined with a Shinadzu TOC Analyzer 500. Based on effect of biomass of test algae, the 72 hrs NOEC, EC10 and EC50 values were determined to be 53, 91 and 255 mg/l respectively. Based on growth rate EC10 an EC50 values were 190 and > 420 mg/l. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence, considered to be not-classified as per CLP classification criteria.

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from a secondary source.

Qualifier:

according to guideline

Guideline:

other: Directive 92/69/EEC, C.3

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance was added to synthetic fresh water to provide a concentration of 1g/l and stirred for 18 hours. The solution was filtered and the TOC content determined. This solution serves as the primary dilution of the test substance.

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Test temperature:

24°C

Nominal and measured concentrations:

Nominal concentrations in mg/L in Main test 2: 0, 33, 67, 133, 278, 556, and 1000 mg/L.
Nominal concentrations in mg/L in Main test 4: 1.3, 2.5, 5.1, and 10.1.

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: <1mg/L

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Other details not known

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

321 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Other details not known

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

38 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Other details not known

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

603 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: Other details not known

Validity criteria fulfilled:

not specified

Conclusions:

Based on toxicity to growth rate of test alage, the 72 hrs NOEC, EC10 and EC50 values were determined to be 1.3, 38 and 603 mg/l respectively.

Executive summary:

Toxicity to algae study was carried out for time period of 72 hrs by following the guideline OECD 201, Directive 92/69/EEC, C.3. Study was performed unser static condition. Scenedesmus subspicatus was used as test organism. Test was carried out at temperature of 24°C of TOC <1mg/L. Verification was done by using TOC-500 Infrared Analyzer. Test substance was added to synthetic fresh water to provide a concentration of 1g/l and stirred for 18 hours. The solution was filtered and the TOC content determined. Based on effect of growwth rate of test algae NOEC, EC10 and EC50 values were determined to be 1.3, 38 and 603 mg/l respectively. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence considered to be not classified as per CLP classification criteria.

Reference 3

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

calculation (if not (Q)SAR)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: Modeling database

Principles of method if other than guideline:

Estimation 96 hrs EC50 value of test chemical on aquatic algae by EPI Suite ECOSAR version 1.11.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: green algae

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Remarks on exposure duration:

Estimated data

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: EC50 value was determined to be > 100 mg/l (i.e 95897 mg/l)

Validity criteria fulfilled:

not specified

Conclusions:

Based on effect of test chemical on the growth rate of the test organism green algae, the 96 hrs EC50 value was estimated to be >100 mg/l (i.e 95897 mg/l)

Executive summary:

The toxicity of the test chemical to green algae was predicted using EPI Suite ECOSAR version 1.11. On the basis of effect of test chemical observed in a static system on the growth rate of the test organism during the 96 hr exposure duration, the median effect concentration (EC50) for the test chemical was estimated to be >100 mg/l (i.e 95897 mg/l). Thus, based on the EC50 value, test chemical can be considered as non-toxic to aquatic algae and hence, considered to be 'not classified' as per the CLP classification criteria.

Description of key information

Based on effect of test chemical on the growth rate of the test organism green algae, the 96 hrs EC50 value was estimated to be >100 mg/l (i.e 95897 mg/l)

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L

Additional information

Predicted data of target chemical and supporting weight of evidence studies for it's  structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on algae. The studies are as mentioned below:

 

First study includes the toxicity of the test chemical to green algae was predicted using EPI Suite ECOSAR version 1.11. On the basis of effect of test chemical observed in a static system on the growth rate of the test organism during the 96 hr exposure duration, the median effect concentration (EC50) for the test chemical was estimated to be >100 mg/l (i.e 95897 mg/l). Thus, based on the EC50 value, test chemical can be considered as non-toxic to aquatic algae and hence, considered to be 'not classified' as per the CLP classification criteria.

 

Seond study includes toxicity to algae study was carried out for time period of 72 hrs by following the guideline OECD 201. Study was performed unser static condition. Scenedesmus subspicatus was used as test organism. Test was carried out at temperature of 24°C of TOC <1mg/L. Verification was done by using TOC-500 Infrared Analyzer. Test substance was added to synthetic fresh water to provide a concentration of 1g/l and stirred for 18 hours. The solution was filtered and the TOC content determined. Based on effect of growwth rate of test algae NOEC, EC10 and EC50 values were determined to be 1.3, 38 and 603 mg/l respectively. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence considered to be not classified as per CLP classification criteria.

 

Third study includes toxicity to algae study was carried out for time period of 72 hrs by following the method of Directive 92/69/EEC, C.3. Study was performed under static condition at temperature 24 +/- 2 deg C. Scenedesmus subspicatus was used as test organism. Test medium used was as described in guideline 92/69/EEC C.3. (1992). Stock solution was prepared as1 g/l of the test item was equilibrated in deionized water, stirred for 18 hours, and filtered. Green algae were exposed at concentrations of 0, 29, 53, 84, 147, 252, and 420 mg/l. Light intensity used was 8000 lux, white. pH at beginning of test 6.9-7.6 and end of test was 8.3-8.9 respectively. sterile-aerated Erlenmeyer flasks was used as test vessel. The analyses were carried out by measurement of dissolved organic carbon. The DOC content was determined with a Shinadzu TOC Analyzer 500. Based on effect of biomass of test algae, the 72 hrs NOEC, EC10 and EC50 values were determined to be 53, 91 and 255 mg/l respectively. Thus, based on EC50 value test chemical was considered as non-toxic to test algae and hence, considered to be not-classified as per CLP classification criteria.

 

Based on effect of test chemical on the growth rate of the test organism green algae, the 96 hrs EC50 value was estimated to be >100 mg/l (i.e 95897 mg/l).

 

Based on above information test chemical was considered as non-toxic to test algae and hence, considered to be not-classified as per CLP classification criteria.