Dihydrogen [[N,N'-ethylenebis[N-(carboxymethyl)glycinato]](4-)-N,N',O,O',ON,ON']nickelate(2-)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Remarks:

Experimental data of read across substances

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data for the target chemical is summarized based on the read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

no guideline available

Principles of method if other than guideline:

WoE report is based on two toxicity to microorganisms studies as-
WoE 2 and WoE 3.

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

not specified

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

WoE 2: Pseudomonas putida

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Remarks on exposure duration:

3 to 16 hr

Reference substance (positive control):

not specified

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 105 - < 403 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: EC50 value of test chemical was found to be in range of > 105 to < 403 mg/l.

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism. EC50 value of test chemical was found to be in range of > 105 to < 403 mg/l in duration of 3 hr to 16 hr.

Executive summary:

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on microorganisms. The studies are as mentioned below:

The first study includes toxicity to microorganisms was carried out. Duration of study was 16 hr. Pseudomonas putida was taken as test organisms. As per the toxicity to microorganisms study the 96 hr EC0 and EC50 value was determined to be 105 mg/l and > 105 mg/l respectively.

The second study includes study of toxicity to microorganisms was carried out in accordance with OECD 209. The duration of the study was 3 hr.  Source of test chemical was Akzo Nobel Chemicals b.v. Amersfoort. Activated sludge of predominantly domestic sewage was taken as test organism. On the basis of toxicity to microorganisms study the 3 hr EC10, EC20 and EC50 value was determined to be 48 mg/l, 105 mg/l and 403 mg/l (with 95% CI value of 227 to 597 mg/l) respectively. 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism. EC50 value of test chemical was found to be in range of > 105 to < 403 mg/l in duration of 3 hr to 16 hr.

Description of key information

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism. EC50 value of test chemical was found to be in range of > 105 to < 403 mg/l in duration of 3 hr to 16 hr.

Key value for chemical safety assessment

EC50 for microorganisms:

105 mg/L

Additional information

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on microorganisms. The studies are as mentioned below:

The first study includes toxicity to microorganisms was carried out. Duration of study was 16 hr. Pseudomonas putida was taken as test organisms. As per the toxicity to microorganisms study the 96 hr EC0 and EC50 value was determined to be 105 mg/l and > 105 mg/l respectively.

The second study includes study of toxicity to microorganisms was carried out in accordance with OECD 209. The duration of the study was 3 hr.  Source of test chemical was Akzo Nobel Chemicals b.v. Amersfoort. Activated sludge of predominantly domestic sewage was taken as test organism. On the basis of toxicity to microorganisms study the 3 hr EC10, EC20 and EC50 value was determined to be 48 mg/l, 105 mg/l and 403 mg/l (with 95% CI value of 227 to 597 mg/l) respectively. 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism. EC50 value of test chemical was found to be in range of > 105 to < 403 mg/l in duration of 3 hr to 16 hr.