Dihydrogen [[N,N'-ethylenebis[N-(carboxymethyl)glycinato]](4-)-N,N',O,O',ON,ON']nickelate(2-)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

Experimental data of read across substances

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data for the target chemical is summarized based on the read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

no guideline available

Principles of method if other than guideline:

WoE report is based on three short term toxicity to aquatic invertebrates studies as-
WoE 2, WoE 3 and WoE 4.

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

WoE 3- Concentrations: 0, 25, 50, 100, 200, 400, 800 mg/L
WoE 2, WoE 4: No data available

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

Common Name: Water flea

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

WoE 3: 20 ± 1 °C

pH:

WoE 3: 6.8-7.0

Dissolved oxygen:

WoE 3: 6.5 mg/L

Nominal and measured concentrations:

WoE 2, WoE 4: No data available
WoE 3: 0, 25, 50, 100, 200, 400, 800 mg/L

Details on test conditions:

WoE 2, WoE 4: No data available
WoE 3: TEST SYSTEM
- Test vessel: Glass Beaker
- Type (delete if not applicable): open
- Volume of solution: 100 ml
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 12
- No. of vessels per concentration (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: 6.8-7.0 with 1 M NaOH
- Photoperiod: 16 hr light with 8 hr darkness

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 - < 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Test chemical was found to be non toxic.

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.

Executive summary:

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:

The first study of short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be 113 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

The second study was of short-term toxicity to aquatic invertebrates. The duration of test was 48 hr performed under semi static conditions. Daphnia carinata was taken as a test organism. The test vessel was taken as 100 ml glass beaker.  Renewal rate of test solution was done daily. Number of organisms per vessel was taken as 12. Number of vessels per concentration (replicates) was taken as four. Test conditions includes temperature of 20 ± 1 °C, adjustment of pH 6.8-7.0 was done with 1 M NaOH and dissolved oxygen was of 6.5 mg/L. Photoperiod of 16 hr light with 8 hr darkness was provided. Test concentration includes 0, 25, 50, 100, 200, 400, 800 mg/L. Probit analysis was used to calculate the 24-h, 48 hr EC50. On the basis of mortality effect of test chemical on test organism Daphnia carinata the 24, 48 hr EC50 value was determined to be 342.9 mg/L, with 95% CI of 168.5 to 678.6 mg/l and 245.0 mg/L with 95% CI of 200 to 400 mg/l respectively.

The third study includes short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

Description of key information

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 - < 400 mg/L

Additional information

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:

The first study of short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be 113 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

The second study was of short-term toxicity to aquatic invertebrates. The duration of test was 48 hr performed under semi static conditions. Daphnia carinata was taken as a test organism. The test vessel was taken as 100 ml glass beaker.  Renewal rate of test solution was done daily. Number of organisms per vessel was taken as 12. Number of vessels per concentration (replicates) was taken as four. Test conditions includes temperature of 20 ± 1 °C, adjustment of pH 6.8-7.0 was done with 1 M NaOH and dissolved oxygen was of 6.5 mg/L. Photoperiod of 16 hr light with 8 hr darkness was provided. Test concentration includes 0, 25, 50, 100, 200, 400, 800 mg/L. Probit analysis was used to calculate the 24-h, 48 hr EC50. On the basis of mortality effect of test chemical on test organism Daphnia carinata the 24, 48 hr EC50 value was determined to be 342.9 mg/L, with 95% CI of 168.5 to 678.6 mg/l and 245.0 mg/L with 95% CI of 200 to 400 mg/l respectively.

The third study includes short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.