Dihydrogen [[N,N'-ethylenebis[N-(carboxymethyl)glycinato]](4-)-N,N',O,O',ON,ON']nickelate(2-)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Remarks:

Experimental data of read across substances

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data for the target chemical is summarized based on the read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

no guideline available

Principles of method if other than guideline:

WoE report is based on three studies of toxicity to aquatic algae as-
WoE 2, WoE 3 and WoE 4.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: WoE 2: microcystis aeruginosa, WoE 3: scenedesmus capricornutum, WoE 4: Pseudokirchnerella subcapitata

Details on test organisms:

Common Name: Geen algae

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Remarks on exposure duration:

72 hr to 8 days

Nominal and measured concentrations:

WoE 2, WoE 3: No data available
WoE 4: 60, 80, and 100 mg/l

Reference substance (positive control):

not specified

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Test chemical was found to be non toxic.

Validity criteria fulfilled:

not specified

Conclusions:

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of toxicity to green algae the 72 hr EC50 value was determined to be > 100 mg/l.

Executive summary:

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic algae. The studies are as mentioned below:

First study was of toxicity to aquatic algae. Duration of study was 8 days. Microcystis aeruginosa a freshwater cyanobacteria was taken as test organism. On the basis of toxicity to freshwater cyanobacteria ( Microcystis aeruginosa) the 8 days EC0 and EC50 value was determined to be 76 mg/l and > 76 mg/l respectively. 

Second study includes of toxicity to aquatic algae study. Duration of study was 72 hr.  Selenastrum capricornutum a freshwater green algae was taken as test organism. On the basis of toxicity to green algae (Selenastrum capricornutum) the 72 hr EC50 value was determined to be > 72.7 mg/l. 

Study of toxicity to aquatic algae was carried out in accordance with OECD 201. Duration of study was 72 hr. Green algae Pseudokirchnerella subcapitata, was taken as test organism. Nominal concentrations of 60, 80, and 100 mg/l were used, during the test the concentrations were measured by HPLC. On the basis of toxicity to growth rate of green algae Pseudokirchnerella subcapitata, 72 hr NOEC and EC50 value was determined to be 79. 4 and > 100 mg/l respectively. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of toxicity to green algae the 72 hr EC50 value was determined to be > 100 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

Description of key information

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of toxicity to green algae the 72 hr EC50 value was determined to be > 100 mg/l.

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L

Additional information

Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic algae. The studies are as mentioned below:

First study was of toxicity to aquatic algae. Duration of study was 8 days. Microcystis aeruginosa a freshwater cyanobacteria was taken as test organism. On the basis of toxicity to freshwater cyanobacteria ( Microcystis aeruginosa) the 8 days EC0 and EC50 value was determined to be 76 mg/l and > 76 mg/l respectively. 

Second study includes of toxicity to aquatic algae study. Duration of study was 72 hr.  Selenastrum capricornutum a freshwater green algae was taken as test organism. On the basis of toxicity to green algae (Selenastrum capricornutum) the 72 hr EC50 value was determined to be > 72.7 mg/l. 

Study of toxicity to aquatic algae was carried out in accordance with OECD 201. Duration of study was 72 hr. Green algae Pseudokirchnerella subcapitata, was taken as test organism. Nominal concentrations of 60, 80, and 100 mg/l were used, during the test the concentrations were measured by HPLC. On the basis of toxicity to growth rate of green algae Pseudokirchnerella subcapitata, 72 hr NOEC and EC50 value was determined to be 79. 4 and > 100 mg/l respectively. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria. 

On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of toxicity to green algae the 72 hr EC50 value was determined to be > 100 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.