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EC number: 696-026-0
CAS number: 1395383-69-3
Two studies were performed to determine the acute toxicity of bis(2,3-epoxypropyl)cyclohexane-1,2-dicarboxylate to fish.The first study was performed with diglycidyl-1,2-cyclohexane to Fathead minnow (Pimephales promelas Rafinesque) in a 96-hour Static Test according to OECD 203 and EPA OTS 797.1400 and the second study was performed on Carp (Cyprinus carpio) in a 96-hour Static test according to OECD 203 and EU Method C.1.
In a first study the test substance was test for its acute toxicity to
fish. The study was designed as a 96h static acute toxicity test with
fathead minnow, Pimephales promelas Rafinesque. Ten fish per
concentration were exposed to nominal concentrations of 7, 12, 20, 34
and 50 mg/L of the test substance, an acetone control and a laboratory
The 96h LC50 for the test substance was determined to be 17.0 mg/L. The
96h mortality threshold concentration was 12 mg/L and the no observed
concentration (NOEC) was 7.0 mg/L.
In the second study a range-finding test was performed and a LC50 test
was performed with carp exposed to concentrations ranging from 10 to 100
mg/l in a static system, increasing with a factor of 1.8. Acetone was
used as a sol vent in the stock solutions. Based on the results of this
test, the 96h-LC50 for carp was between 32 and 56 mg/l with 0 %
mortality at 18 mg/l, 10 % mortality at 32 mg/l and 100 % mortality at
56 mg/l. The definitive test was performed with carp exposed to
concentrations ranging from 20 to 56 mg/l in a static system, increasing
with a factor of 1.3. Based on the results of the definitive test, the
96h-LC50 was 35 mg/l (95 % confidence interval between 33 and 40 mg/l).
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