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Short-term toxicity to fish

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Description of key information

The acute toxicity of alpha-methylstyrene to Brachydanio rerio was investigated in a study conducted according to EU Method C.1 (Acute Toxicity for Fish; 2008) and OECD Guideline 203 (Fish, Acute Toxicity Test; 1992). The 96 h LC50, NOEC and LOEC were determined to be 2.97, 2.13, and 3.19 mg/L based on measured concentrations (geom. mean).

Key value for chemical safety assessment

LC50 for freshwater fish:
2.97 mg/L

Additional information

Reliable results obtained in a guideline studies are available. The acute toxicity of alpha-methylstyrene to Brachydanio rerio was investigated in a study conducted according to EU Method C.1 (Acute Toxicity for Fish; 2008) and OECD Guideline 203 (Fish, Acute Toxicity Test; 1992). 7 Fish were exposed under static conditions to nominal concentrations of 0 (control), 10, 20, 40, and 80 mg/L nominal (WAF) corresponding to initial measured concentrations of 0 (control), 2.613, 4.552, 10.164, and 30.527 mg/L (WAF). The test substance concentrations were determined via GC/FID. After 96 h, the test substance concentrations had decreased to concentrations < 1 mg/L. Therefore the calculation as geometric mean of t (0 h) vs. t (96 h) is not appropriate. The 96 h LC50, NOEC and LOEC were determined to be 2.97, 2.13, and 3.19 mg/L based on measured concentrations (geom. mean). The validity criteria given by the guidelines were fulfilled (control: no dead animals were observed; DO>=60% of air saturation value).

The acute toxicity of alpha-methylstyrene to Oryzias latipes was investigated in a study conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). 10 Fish were exposed to alpha-methylstyrene at nominal concentrations of 0 (control), solvent control, 3.0, 6.0, 12.0 24.0, and 48.0 mg/L for 96 h under semi-static conditions. The test substance concentrations were determined by HPLC in freshly prepared solutions and in 24 h old test solutions. The geometric mean measured concentrations at concentrations of 3-12 mg/L nominal were in the range between 78 and 85% of the initial nominal concentrations. Due to the death of fish within 24 h at concentration levels >=24 mg/L, the calculation of the mean geometric concentrations was not performed. A 96 h LC50=7.28 mg/L

(geometric mean measured) was determined.

The prolonged toxicity of alpha-methylstyrene to Oryzias latipes was investigated in a study conducted according to OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study). 10 Fish were exposed to alpha-methylstyrene at nominal concentrations of 0 (control), solvent control, 0.5, 1.3, 3.1, and 8 mg/L for 14 d under flow-through conditions. The test substance concentrations were monitored via HPLC/UV. A 14 d LC50>6.78 mg/L was determined based on mean measured concentrations. The 14 d NOEC (growth: length and weight of fish) was determined to be 1.04 mg/L. Based on these results it can be concluded that the long-term toxicity of fish is comparable to the short-term toxicity.