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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Older study: meets scientific standards with acceptable restrictions (limited number of animals in study, partly limited documentation)

Data source

Reference
Reference Type:
publication
Title:
Toxicological studies of certain alkylated benzenes and benzene. Experiments on laboratory animals.
Author:
Wolf MA, Rowe VK, McCollister DD, Hollingsworth RL & Oyen F
Year:
1956
Bibliographic source:
AMA Arch Ind Health 14, 387 - 398

Materials and methods

Test guideline
Qualifier:
no guideline followed
Guideline:
other: Study from 1956 (no guidelines available at the time the study was performed)
Deviations:
not applicable
GLP compliance:
no
Remarks:
Study from 1956 (GLP was not compulsory at the time the study was performed)
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name of test material (as cited in study report): alpha-Methyl styrene
Purity: 98.6 - 99.1 % (obtained by UV or IR analysis)
Boiling point: 165.4 degrees C
Specific gravity: 0.91 (at 20 degrees C)

Test animals

Species:
rat
Strain:
Wistar
Sex:
male
Details on test animals and environmental conditions:
Source: Wistar Institute
Age at study initiation: young adult
Weight at study initiation: 175 – 250 g
Diet: modified Sherman stock diet

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: The tested materials were applied as undiluted material or as an olive-oil or corn-oil solution emulsified with a 5 – 10 % aqueous solution of gum arabic (not further specified).
Details on oral exposure:
The total volume administered always was <= 7 mL.
Doses:
Not further specified.
No. of animals per sex per dose:
Number of male animals used: 20.
Control animals:
not specified
Details on study design:
All the surviving rats were observed until recovery was assured (usually about 2 weeks).
Necropsy of survivors performed: yes.
Statistics:
Not further specified.

Results and discussion

Preliminary study:
Not applicable.
Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 4 900 mg/kg bw
Mortality:
Not further specified.
Clinical signs:
Not further specified.
Body weight:
Not further specified.
Gross pathology:
In autopsied rats slight liver changes and, in some instances, some kidney changes of questionable significance were observed (these effects were described for all tested substances without further specification).

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Remarks:
Migrated information
Conclusions:
This study (published in 1956) summarises the results of several individual tests with different chemicals. With an oral LD50 value of ca. 4900 mg/kg bw in male rats, the substance is practically nontoxic.
Executive summary:

In this study performed in the year 1956, male Wistar rats were dosed orally once via gavage with a post-exposure of 14 days. The obtained LD50 value was ca. 4900 mg/kg bw and in autopsied rats slight liver changes and, in some instances, some kidney changes of questionable significance were observed.

Although the documentation of this study in some parts is limited, the main results have been reported.