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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Repeated dose toxicity: dermal

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Administrative data

Endpoint:
short-term repeated dose toxicity: dermal
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Only local effects were evaluated. Same animals were treated with different dose level at week 1, 2, and 3. Non-GLP. Non-standard test method. No purity information provided.

Data source

Reference
Reference Type:
publication
Title:
Histopatholgical and ultrastructural effects of glycolic acid on rat skin
Author:
Inan S, Oztukcan S, Vatansever S, Ermertcan AT, Zeybek D, Oksal A, Giray G, Muftuoglu S
Year:
2006
Bibliographic source:
Acta Histochemica, 108: 37-47.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The effects of glycolic acid applied in different dosages on rat skin was evaluated using light and electron microscopy.
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Glycollic acid
EC Number:
201-180-5
EC Name:
Glycollic acid
Cas Number:
79-14-1
Molecular formula:
C2H4O3
IUPAC Name:
2-hydroxyacetic acid
Details on test material:
- Name of test material (as cited in study report): glycolic acid

Test animals

Species:
rat
Strain:
Wistar
Sex:
not specified

Administration / exposure

Type of coverage:
open
Vehicle:
water
Analytical verification of doses or concentrations:
no
Duration of treatment / exposure:
up to 3 weeks
Frequency of treatment:
No details provided
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
8%
Basis:
other: nominal, in week 1
Remarks:
Doses / Concentrations:
50%
Basis:
other: nominal, in week 2
Remarks:
Doses / Concentrations:
70%
Basis:
other: nominal, in week 3
No. of animals per sex per dose:
5-6
Control animals:
yes

Results and discussion

Results of examinations

Histopathological findings: non-neoplastic:
effects observed, treatment-related

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

In the group treated with 8%, H&E staining showed mild basal cell activation. Epidermal thickness had increased statistically in comparison with the control group. Immunohistochemistry revealed moderate immunoreactivity of type 1 collagen in the dermis.

Applicant's summary and conclusion

Conclusions:
Histochemical and immunohistochemical observations demonstrated that glycolic acid treatment resulted in statistically significant increased thickness of the epidermis and an increase in the numbers of active fibroblasts and in the amount of dense collagen, especially at higher doses. Cytoplasmic vacuolization in epidermal keratinocytes, intercellular disjunction, and increased quantities of organized bundles of collagen fibers in the dermis were observed in ultrastructural examination of rat skin from treated animals.