Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Version / remarks:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Source: sewage treatment plant Ruhrverband Kläranlage, Schmallenberg, Germany; kept aerobic until use; conc. used in test: 29.6 mg dry mass/l (= 7.39 mg dry mass/250 ml)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
31
Sampling time:
28 d
Details on results:
biodegradation within 10-day window: 21%

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
test item not readily biodegradable under the chosen test conditions