Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
Source: SAG, Culture Collection of Algae at Pflanzenphysiologisches Institut of the University at Göttingen, Albrecht von Haller Institut, Untere Klarspüle 2, 37073 Göttingen, germany. Catalogue: no. 61.81

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
none

Test conditions

Hardness:
A sterilized synthetic growth medium (OECD medium) according to OECD 201 was used for culture and preparation of the test medium.
Test temperature:
21.5-22.0°C
pH:
7.45-8.03
Dissolved oxygen:
-
Salinity:
-
Nominal and measured concentrations:
Nominal: 6.25, 12.5, 25, 50 and 100 mg/l; measured: all < 0.35 mg/l
Details on test conditions:
test vessel: 250 ml conicalglass flasks
type of vessel: covered with silicone-sponge caps (sterilized prior to use)
Initial cells' density: 10,000 cells/ml
Number of organisms per vessel: 1,000,000
Number of vessels per concentration: 3 replicates
Number of vessels per control: 6 replicates
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for growth rate and yield was > 100 m/l based on nominal loading. The NOEC was determined to be >= 100 mg/l (nominally based). Basedon measured concentrations, the values EC50 values were > 0.35 mg/l, and the NOEC values were >= 0.35 mg/l.