Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test solutions

Vehicle:
no
Details on test solutions:
Eldew PS-308 is very viscous and of poor water solubility; test solutions were prepared in dilution water to obtain the maximum dissolved concentration (according to OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures); 200 mg Eldew PS-308 were given into 2 l dilution water (2-l flask) for 48 hrs under stirring; after 48 hrs time was given for 1 hr to potential droplets to move to the surface; then 200 ml wee discarded; the following 500 ml of the test item preparation were used for the test (as stock solution without filtration)

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
clone V, 4-24 hrs old at test start

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
80 mg/l as CaCO3 hardness (this value < recommended range (140-250 mg/l as CaCO); however, lower hardness is accepted by guideline)
Test temperature:
20.2-20.3°C
pH:
7.7 - 8.7
Dissolved oxygen:
7.7-8.9 mg/l
Salinity:
see hardness
Nominal and measured concentrations:
nominal: 6.25. 12.5, 25, 50, 100 mg/l
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The NOEC is >= 100 mg ELDEW PS-308 per liter nominal loading.
The EC50 is assumed to be > 100 mg ELDEW PS-308 per liter nominal loading, corresponding to water solubility under test conditions (up to 0.35 mg/l).