[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Test solutions

Vehicle:

no

Details on test solutions:

Eldew PS-308 is very viscous and of poor water solubility; test solutions were prepared in dilution water to obtain the maximum dissolved concentration (according to OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures); 200 mg Eldew PS-308 were given into 2 l dilution water (2-l flask) for 48 hrs under stirring; after 48 hrs time was given for 1 hr to potential droplets to move to the surface; then 200 ml wee discarded; the following 500 ml of the test item preparation were used for the test (as stock solution without filtration)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

clone V, 4-24 hrs old at test start

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

80 mg/l as CaCO3 hardness (this value < recommended range (140-250 mg/l as CaCO); however, lower hardness is accepted by guideline)

Test temperature:

20.2-20.3°C

pH:

7.7 - 8.7

Dissolved oxygen:

7.7-8.9 mg/l

Salinity:

see hardness

Nominal and measured concentrations:

nominal: 6.25. 12.5, 25, 50, 100 mg/l

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC is >= 100 mg ELDEW PS-308 per liter nominal loading.
The EC50 is assumed to be > 100 mg ELDEW PS-308 per liter nominal loading, corresponding to water solubility under test conditions (up to 0.35 mg/l).