Ibuprofen

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Similar to guideline study with acceptable restrictions (e.g. no analytical monitoring); basic data given.

Data source

Reference

Reference Type:

publication

Title:

Aquatic ecotoxicity of pharmaceuticals including the assessment of combination effects

Author:

Cleuvers M

Year:

2004

Bibliographic source:

Toxicol Letters 142: 185-194

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

cited as European Guideline (Comission of the European Communities, 1993)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 2-(4-isobutylphenyl)propionate

EC Number:

250-477-6

EC Name:

Sodium 2-(4-isobutylphenyl)propionate

Details on test material:

- Name of test material (as cited in study report): sodium salt of ibuprofen (alpha-methyl-4-[isobutyl]phenylacetid acid)
- Purity: analytical grade substance

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: Chodat, SAG 86.81 = UTEX 2594
- Source (laboratory, culture collection): SAG-Collection of Agricultures, University of Göttingen, Germany

ACCLIMATION
- Culturing media and conditions (same as test or not): yes

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Remarks on exposure duration:

following guideline (C.3)

Test conditions

Test temperature:

approx. 23 ±2°C

Nominal and measured concentrations:

nominal test concentration: 1, 3.2, 10, 32, 100, and 320 mg/L

Details on test conditions:

OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity and quality: white light (120µEs^-1m^-2)
- Initial cells density: 10^4 cells/mL

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

342.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Exceeding the substance's water solubility limit.

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

102.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Exceeding the substance's water solubility limit.