Ibuprofen

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Screening test

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Principles of method if other than guideline:

Screening test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ibuprofen

EC Number:

239-784-6

EC Name:

Ibuprofen

Cas Number:

15687-27-1

Molecular formula:

C13H18O2

IUPAC Name:

2-(4-isobutylphenyl)propanoic acid

Details on test material:

- Name of test material (as cited in study report): Ibuprofen
- Lot. No.: SN 9815; purity: tested as whole material; water solubility: 15.8 g/L

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test concentrations were prepared by adding 2.4 ml of an appropriate stock solution (prepared in DMF) or the required neat test substance and 2.4 ml of DMF directly into the dilution water within each test chamber and stirring. The solvent concentration in all test chambers was 100 µl/L.
- Chemical name of vehicle: DMF

Test organisms

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

Body length: 29 - 33 mm
Body weight: 0.31 - 0.71 g

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

19.9 - 21.2 °C

pH:

6.1 - 6.9

Dissolved oxygen:

Oxygen content before the test: 9.0 - 9.2 mg/L
Oxygen content after the test: >= 3.3 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 3, 10, 30, 100 and 300 mg/L

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Test water: treated Jupiter Town water
- Hardness as CaCO3: 44 mg/L
- Alkalinity as CaCO3: 11 mg/L
- Conductivity: 311 µS/cm
- pH: 7.1 - 7.4

TEST SYSTEM
- Test vessels: All glass aquaria (42 L)
- Volume of water: 24 L
- No. of animals per test concentration and control: 10
- Loading rate (g fish/L test water): 0.24

- Observation time: 0, 24, 48, 72 and 96 h.
- Endpoints investigated: mortality and symptoms of intoxication.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

173 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality of Lepomis macrochirus, exposed to ibuprofen for 96 hours under static test conditions

Nominal concentration [mg/L]	Cumulative Mortality [%]
	24 h	48 h	72 h	96 h
0 (control)	0	0	0	0
0 (solvent control)	0	0	0	0
3	0	0	0	0
10	0	0	0	0
30	0	0^b	0^b	0^e
100	0^a	0^b	0^c	10
300	0^a	0^b	0^d	90^f

^aUndissolved chemical present.

^bCemical film on water surface.

^cOne fish exhibited a partial loss of equilibrium and was observed at the surface.

^dOne fish exhibited a partial loss of equilibrium; one fish exhibited a complete loss of equilibrium; seven fish had dark pigmentation compared to control fish.

^eOne fish exhibited a partial loss of equilibrium.

^fOne fish exhibited a complete loss of equilibrium.