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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions
Cross-reference
Reason / purpose:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
other company data
Title:
Unnamed
Year:
1966
Report Date:
1966
Reference Type:
secondary source
Title:
SIDS Initial Assessment Report - 2-Methylbut-3-yn-ol
Author:
BASF AG
Year:
2002
Bibliographic source:
OECD SIDS
Reference Type:
secondary source
Title:
Unnamed
Year:
2000

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Principles of method if other than guideline:
Internal methods which were in large part similar to the methods described in OECD Guideline 401
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Analytical purity: 98.5%

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: mean 242 g (male), 185 g( female)

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 2, 16, 20%
- Amount of vehicle (if gavage): 10 ml/kg bw
Doses:
0.2, 1.25, 1.6, 2.00 ml/kg bw (= ca. 172, 1076, 1377, 1722 mg/kg bw)
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and mortality

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1.65 mL/kg bw
Sex:
male/female
Dose descriptor:
LD50
Effect level:
1 420 mg/kg bw
Mortality:
2.000 ml/kg bw: 8/10 within 48 h; 10/10 within 7 days
1.60 ml/kg bw: 4/10 within 24 h
1.25 ml/kgbw : 1/10 within 24 h
0.20 ml/kg bw: 0/10 within 7 days
Clinical signs:
1.60 -2.00 ml/kg bw:
- shortly after application: staggering, apathy, dyspnea, abdominal and lateral position,
- after 10 -30 minutes: narcosis
- after 5 h: narcosis, discharge of the eyes
- 48 h: abdominal and lateral position, narcosis
- days 3-4: intermittent respiration, apathy, piloerection, red crusted eyes
- days 5-6: no effects

1.25 ml/kg bw:
- shortly after application: staggering, apathy, dyspnea, abdominal and lateral position,
- after 10 -30 minutes: temporal narcosis
- after 5 h: narcosis, discharge of the eyes
- up to 48 h: intermittent respiration, apathy, piloerection, red crusted eyes
- after 6 days: no effects

0.20 ml/kg bw:
- up to 24 h: abdominal and lateral position, apathy
- later no effects
Gross pathology:
- 3x hydrothorax, collapse of lung, blood in renal capsule, blood in urine at 2.0 ml/kg bw
- organs: no effects

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU