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EC number: 249-044-4
CAS number: 28472-97-1
Table. Percentage Biodegradation Values
* Day 29 values corrected to include any carry-over of CO2 detected in
Table. Total and Inorganic Carbon Values in the Culture Vessels on Day 0
IC Content (')/0 of TC)
10 mg C/L R1
10 mg C/L R2
R1 - R2 = Replicates 1 and 2
* Corrected for control values. Negative values are due to measured
concentrations being less than control values
** Total carbon value given is the sum of the TC value obtained from
analysis and the nominal TC contribution of the test item
Table. pH Values of the Test Preparations an Days 0 and 28
Inoculum Control R1
Inoculum Control R2
Procedure Control R1
Procedure Control R2
Test Item R1
Test Item R2
Readily biodegradable: 72% (CO2 evolution) in 28 days
One study is available assessing the ready biodegradability of
Diisodecyl azelate (CAS 28472-97-1) in an aerobic aqueous medium
(Harlan, 2014). The method followed was designed to be compatible
with the OECD Guidelines for Testing of Chemicals (1992) No. 301B,
"Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C
of Commission Regulation (EC) No. 440/2008.
The test item, at a concentration of 10 mg carbon/L, was exposed to
activated sewage sludge micro-organisms with mineral medium in sealed
culture vessels in the dark at 20 to 22 °C for 28 days. Following the
recommendations of the International Standards Organisation (ISO 1995)
and the published literature (Handley et al, 2002), the test item was
adsorbed onto granular silica gel prior to dispersion in the test medium
to aid dispersion of the test item in the test medium and to increase
the surface area of the test item exposed to the test organisms. The
biodegradation of the test item was assessed by the determination of
carbon dioxide produced. Control solutions with inoculum and the
reference item, sodium benzoate, together with a toxicity control were
used for validation purposes.
The test item attained 72% biodegradation after 28 days and satisfied
the 10-Day window validation criterion, whereby 60% biodegradation must
be attained within 10 days of the biodegradation exceeding 10%. The test
item can therefore be considered to be readily biodegradable under the
strict terms and conditions of OECD Guideline No. 301B.
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