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Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
not specified
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Presence and estrogenicity of anthracene derivates in coastal Japanese waters
Author:
Ryo Kurihara et al.
Year:
2005
Bibliographic source:
Environmental Toxicology and Chemistry, Vol. 24, No. 8, pp. 1984–1993, 2005

Materials and methods

Type of study / information:
anthracene and hydroxy-anthraquinones (hATQs) for estrogenic activity (Endocrine disruptor)
Test guideline
Qualifier:
no guideline followed
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
97% pure

Results and discussion

Applicant's summary and conclusion

Conclusions:
In an estrogenicity assay system with the yeast two-hybrid system 1,2-dihydroxyaanthraquinone showed estrogen agonist activity,
Executive summary:

In this study the yeast two-hybrid system was used to evaluate the estrogenic activity of hydroxy-anthraquinones. Estrogen agonist and antagonist tests were carried out with or without rat liver S9.

A receptor-binding assay, a Michigan Cancer Foundation-7 (MCF-7) cell proliferation assay (estrogen screen, E-screen), and reporter gene assays in animal cell lines was used to evaluate estrogenic activity.

1,2-dihydroxyaanthraquinone showed toxicity to yeast especially with rat liver S9 than with no S9. In this estrogenicity assay system with the yeast two-hybrid system 1,2-dihydroxyaanthraquinone showed estrogen agonist activity.