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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
fish embryo acute toxicity (FET)
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
Not specified in publication
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 236 (Fish embryo acute toxicity (FET) test)
Deviations:
yes
Remarks:
test concentrations not assessed
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Specific details on test material used for the study:
no additional information available.

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
No analytical verification of test concentrations done

Test solutions

Vehicle:
yes
Remarks:
DMSO
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Stock solutions: 50, 10, 2, 0.4, 0.08 mM
- Exposure concentrations: 1:100 dilution in E2 embryo medium - Chemical name of vehicle: DMSO, final concentration 0.1 %

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish
- Strain: wild type 5D strain (ahr2-)
- Source:Sinnhuber Aquatic Resaerch Laboratory
- Collection of embryos: following group spawnin of adult zebrafish
- Breeding conditions: water temperature 28°C, 14h light, 10 h dark photoperiod, recirculating system

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
120 h
Remarks on exposure duration:
120 hours post fertilization

Test conditions

Nominal and measured concentrations:
Nominal: 0.8, 4, 20, 100 and 500 μM
Details on test conditions:
TEST SYSTEM
- Test vessel: 96 well plates
- No. of organisms per vessel: one embryo per well - No. of vessels per concentration (replicates): 2
- No. vehicle control: 1% DMSO: 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: E2 embryo medium

EFFECT PARAMETERS MEASURED
- Developmental progress
- Somite
- Notochord malformations
- mortality
- RNA
- Gene expression

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 5
- Test concentrations: 0.8, 4, 20, 100 and 500 μM
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
120 h
Dose descriptor:
EC50
Effect conc.:
185 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: total mortality120 hpf
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
300 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: mortality 24 hpf
Duration:
120 h
Dose descriptor:
EC50
Effect conc.:
45 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks on result:
other: body axis and otic

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
The effect of 1,2-dihydroxyanthraquinone on developing zebrafish (embryos) was assessed. The EC50 for mortality was found to be 300 μM 24 hours post fertilization and 185 μM 120 hours post fertilization.
Executive summary:

The effect of 1,2-dihydroxyanthraquinoneon developing zebrafish was assessed in a static test set-up. The test substance was dissolved in DMSO and final nominal test concentrations assessed were: 0.8, 4, 20, 100 and 500 μM. Measurements were done in duplicate with one zebrafish embryo per well. The embryos were evaluated for developmental progress, somite and notochord malformations, and mortality at 24 hpf and total mortality at 120 hpf. Test concentrations were not analytically verified. The EC50 for mortality was found to be 300 μM (= 72 mg/L) 24 hours post fertilization and 185 μM (=44.4 mg/L) 120 hours post fertilization (nominal concentrations).

Observed effects were at such high concentrations that this study was not used in further assessment.