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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 20 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 24hr stirring.After analytical detection the solubility found to be 10 mg/l. Hence, limit test continued with concentration of 10 mg/l.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.63 cm
- Weight at study initiation (mean and range, SD): 0.1899 g (average)
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
148.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.54
Dissolved oxygen:
8.0 mg/l
Nominal and measured concentrations:
10 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 4 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
< 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Table 1. Mortality and symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

-

-

-

-

Table 2: Visible Symptoms

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Nominal

Concentration

(mg/l)

Number of

fish

Temperature (°C) after

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

21

21

25

25

24

24

23

23

100

4

4

21

21

25

25

24

24

23

23

Validity criteria fulfilled:
yes
Conclusions:
Test was performed to determine effect of test chemical on fish Danio rerio. Based on the exposure of test chemical with test organism for 96 hrs, the LC50 was determined to be <10 mg/l.
Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 20 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 24hr stirring.After analytical detection the solubility found to be 10 mg/l. Hence, limit test continued with concentration of 10 mg/l and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Based on the exposure of test chemical with test organism for 96 hrs, the LC50 was determined to be <10 mg/l. Based on above observation test chemical considered to be classified as category chronic-2 according to CLP criteria and thus toxic to aquatic organism

Description of key information

Short term toxicity to fish:

Test was performed to determine effect of test chemical on fish Danio rerio.  Based on the exposure of test chemical with test organism for 96 hrs, the LC50 was determined to be <10 mg/l.

Key value for chemical safety assessment

LC50 for freshwater fish:
10 mg/L

Additional information

Following different studies includes experimental study for the target chemical and read-across analogues which is extracted by using mechanistic approach and functionally and structurally similar to the target chemical to observe the toxicity of test chemical on fish.

First study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was sparingly soluble in water. Therefore, the test solution was prepared by dissolving 20 mg of the test substance in 2 liters of potable water (passed through reverse osmosis system) with 24hr stirring.After analytical detection the solubility found to be 10 mg/l. Hence, limit test continued with concentration of 10 mg/l and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Based on the exposure of test chemical with test organism for 96 hrs, the LC50 was determined to be <10 mg/l. Based on above observation test chemical considered to be classified as category chronic-2 according to CLP criteria and thus toxic to aquatic organism

Second short term fish toxicity study was conducted for 24 hrs for assessing the lethal effect of read across chemical on test organism. Study was conducted in a static system at 18°C and 7.2 pH. Test was perfomed on Ptychocheilus oregonensis as a test organism.On the basis of effect of test chemical on test organism,the 48 hrs LC50 value was determined to be 10mg/l.

Last short term fish toxicity study was conducted for 24 hrs for assessing the lethal effect of read across chemical on test organism. Study was conducted in a static system at 10°C and 7.2 pH. Test was perfomed on Ptychocheilus oregonensis used as a test organism. On the basis of effect of test chemical on test organism, the 24 hrs LC50 value was determined to be 10mg/l.