Registration Dossier

Environmental fate & pathways

Endpoint summary

Administrative data

Description of key information

Biodegradation in water:

The test item  undergoes 46.92 % biodegradation after 42 days in the test condition. Thus, the test item was considered to be ultimate inherently biodegradable.

Additional information

Biodegradation in water:

42-days Closed Bottle test following the OECD guideline 301 D to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used as inoculum at concentration 10E7 to 10E8 CFU/ml. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 4 mg/L, while that of inoculum was 32ml/L. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 72.28 %. Degradation of Sodium Benzoate exceeds 45.18 % on 7 days & 59.03% on 14th day. The activity of the inoculum was thus verified and the test can be considered as valid. The BOD42 value of test substance was observed to be 1.07 mgO2/mg. ThOD was determined by calculation as 2.28 mgO2/mg. Accordingly, the % degradation of the test item after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 46.92 %. Based on the results, the test item under the test conditions was considered to be ultimate inherently biodegradable in nature.