Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24 April 2013 to 7 June 2013
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013
Report Date:
2013

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
in Elendt-M4-medium, pre-aerated for 2 hours

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Test temperature:
20.1 - 20.6 °C
pH:
7.87 - 8.77
Dissolved oxygen:
8.0 - 8.4 mg/L
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
66.2 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
other: EC20
Effect conc.:
174.62 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 400 mg/L
Nominal / measured:
estimated
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC10
Effect conc.:
41.34 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
other: EC20
Effect conc.:
196.67 mg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 400 mg/L
Nominal / measured:
estimated
Conc. based on:
dissolved
Basis for effect:
mobility
Details on results:
The ECx were calculated by probit analysis using linear max. likelihood regression. The EC 50 could not be determined due to mathematical reasons and is reported as range.
Results with reference substance (positive control):
The last quality check was performed in May 2013:
The EC50 (24h) was determined as 2.43 mg/L (95% - confidence interval: n.d.).
The EC50 (48h) was determined as 0.85 mg/L (95% - confidence interval: 0.40 – 1.01 mg/L).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
With the test item Red VP2298 the following result was achieved:
EC50 (24h, nominal concentration): > 400 mg/L
EC50 (48h, nominal concentration): > 400 mg/L.