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EC number: 203-079-1 | CAS number: 103-09-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
There were no informations available for the assessment of the genotoxic potential of 2-EHAc itself. However, there were reliable data available from the structural analogon and metabolite 2-ethylhexan-1-ol to assess the genotoxic potential of 2-EHAc.
In vitro
Gene mutation in bacteria
A GLP conform Ames test was performed with Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 comparable to OECD guideline 471 (TSCATS OTS 0515130, 1987). Doses of up to 5000 µg 2-ethylhexan-1-ol (unknown purity) per plate were dissolved in ethanol and tested with and without S-9 mix as metabolic activation system. 2-ethylhexan-1-ol did not increase the number of revertants in any strain and was therefore not mutagenic in the Ames test. Cytotoxicity and precipitation was observed generally in the highest dose.
Gene mutation in mammalian cells
A GLP conform HGPRT test was performed with Chinese hamster ovary cells (CHO) comparable to OECD guideline 476 (CMA, 1985). Doses of up to 300 or 400 nL 2-ethylhexan-1-ol (unknown purity)/ mL were dissolved in acetone and tested with or without S-9 mix, respectively, as metabolic activation system. 2-ethylhexan-1-ol did not increase the mutant frequencies at the HGPRT locus and was therefore considered as inactive in the CHO/HGPRT test. Cytotoxicity was observed at 400 nL/mL.
Cytogenicity in mammalian cells
A GLP conform UDS test was performed with rat hepatocytes comparable to OECD guideline 486 (TSCATS OTS 0515130, 1987). Doses of up to 1000 nL 2-ethylhexan-1-ol (unknown purity)/ mL were dissolved in DMSO and tested without metabolic activation system. 2-ethylhexan-1-ol did not increase the levels of unscheduled DNA synthesis in rat hepatocytes in the range of 2.5 to 250 nL/mL and was therefore considered as inactive in the UDS test. Cytotoxicity was observed at >= 500 nL/mL.
In vivo
Cytogenicity
A GLP conform micronucleus test was performed in B6C3F1 mice comparable to OECD guideline 474 (CMA, 1982). A dose of 456 mg 2-ethylhexan-1-ol (unknown purity)/ kg bw were dissolved in corn oil and administered i.p. either as single application or as multiple application twice within 24 hours into six animals per sex per treatment. Bone marrow cells were harvested 30 h after the single application or 24 h after the second application. With one exception (multiple treatment males), there was no significant difference in percent micronucleated polychromatic erythrocytes (PCEs) between animals dosed with the test substance and the corresponding negative control. In this treatment, the corresponding negative control in males was very low, and the corresponding negative female control produced comparable percent micronucleated polychromatic erythrocytes (PCEs) as the test substance treated males. Moreover, the percent micronucleated PCEs in the multiple treated males is not very high and in the range of historical control data. Thus, the observed increase was viewed as a statistical artifact with little biological significance. Thus, 2-ethylhexan-1-ol was not considered to be clastogenic under the conditions of this assay.
A GLP conform chromosome aberration assay was performed in Fischer 344 rats comparable to OECD guideline 475 (TSCATS OTS 0515130, 1987). Doses of ca. 16.6, 58.1 and 174.3 mg 2-ethylhexan-1-ol (purity 99.7%)/ kg bw were dissolved in corn oil and administered per gavage on five consecutive days to 5 animals per sex per dose. Bone marrow cells were arrested in C-metaphases by administration of colchicina two hours prior to sacrifice. After examination of 50 metaphases per animal, 2-ethylhexan-1-ol did not cause aberrations in rat bone marrow cells and was therefore considered as inactive under the conditions of this assay.
Read across justification to 2-ethylhexan-1-ol for filling data gaps of 2-ethylhexyl acetate:
As indicated by toxicokinetic studies (see chapter on toxicokinetics, metabolism and distribution), 2-ethylhexyl acetate is rapidly hydrolyzed to 2-ethyhexan-1-ol and acetate (acetic acid). Available data on 2-ethyhexan-1-ol is therefore suitable for filling data gaps of 2-ethylhexyl acetate.
Short description of key information:
in vitro
Gen mutation in bacteria
Ames test, S. typhimurium TA 1535, TA 1537, TA 100, TA 98 with and without metabolic activation: negative (GLP, comp. OECD 471; TSCATS OTS 0515130, 1987)
Gene mutation in mammalian cells
HGPRT test, Chinese hamster ovary cells (CHO) with and without metabolic activation: negative (GLP, comp. OECD 476; CMA, 1985)
Cytogenicity in mammalian cells
UDS test, rat hepatocytes without metabolic activation: negative (GLP, comp. OECD 486; TSCATS OTS 0515130, 1987)
in vivo
Cytogenicity
MNT, i.p. B6C3F1 mouse: negative (GLP, comp. OECD 474; CMA, 1982)
CA, oral F344 rat: negative (GLP, comp. OECD 475; TSCATS OTS 0515130, 1987)
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
According to the available test results for the analogous substance 2-ethylhexan-1-ol, 2-EHAc has not to be classified for genetic toxicity following 67/548/EEC and GHS requirements, respectively.
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