2-ethylhexyl acetate

Repeated dose toxicity: dermal

Administrative data

Endpoint:

short-term repeated dose toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets scientific standards and acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Zur arbeitshygienisch-toxikologischen Beurteilung volkswirtschaftlich bedeutsamer chemischer Substanzen. 4. Kurzmitteilung: Technisches 2-Aethylhexylacetat (Octylacetat).

Author:

Schmidt P, Bachmann W

Year:

1969

Bibliographic source:

Z Gesamte Hyg. 15(12): 928-929

Materials and methods

Principles of method if other than guideline:

Study to assess the subacute dermal toxicity in rats.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhexyl acetate

EC Number:

203-079-1

EC Name:

2-ethylhexyl acetate

Cas Number:

103-09-3

Molecular formula:

C10H20O2

IUPAC Name:

2-ethylhexyl acetate

Details on test material:

2-ethylhexyl acetate, technical purity

Test animals

Species:

rat

Strain:

other: albino

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: own breeding (randomized)
- Weight at study initiation: 163 (160-175) g
- Diet Standard diet K (VEG Altglienecke, Germany) ad libitum
- Water ad libitum

Administration / exposure

Type of coverage:

open

Vehicle:

unchanged (no vehicle)

Details on exposure:

TEST SITE
- Area of exposure: 3*4 cm, shaved skin
- Type of wrap if used: no wrap used; animals were immobilised during exposure


REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

12 days

Frequency of treatment:

twice daily for 2 hours on 10 of 12 days

Doses / concentrations

Doses / concentrations 1

Remarks:

Doses / Concentrations:
0, ca. 1070 mg/kg bw/day
Basis:
nominal per unit body weight

Doses / concentrations 2

Remarks:

Doses / Concentrations:
0, ca. 535 mg/kg bw/treatment
Basis:
nominal per unit body weight

Doses / concentrations 3

Remarks:

Doses / Concentrations:
0, 0.1 ml/animal/ treatment
Basis:
nominal per unit area

No. of animals per sex per dose:

treatment: 12 females; control: 6 females

Control animals:

yes, concurrent no treatment

Details on study design:

Post-exposure period: 28 days

Examinations

Observations and examinations performed and frequency:

The condition of the skin was evaluated, the body weight of the animals was juged twice a week in the beginning, later on once a week.

Sacrifice and pathology:

At the end of the 28 days post exposure, the animals were sacrificed and the organs examined macroscopically.

Results and discussion

Results of examinations

Details on results:

No significant differences were observed between treated and control animals at any time.

Effect levels

1

Dose descriptor:

NOAEL

Remarks:

systemic

Effect level:

>= 1 070 mg/kg bw/day (nominal)

Sex:

female

Basis for effect level:

other: no effects

2

Dose descriptor:

NOAEL

Remarks:

local

Effect level:

>= 1 070 mg/kg bw/day (nominal)

Sex:

female

Basis for effect level:

other: no effects

3

Dose descriptor:

NOAEL

Remarks:

local

Effect level:

>= 14.5 mg/cm² per day (nominal)

Sex:

female

Basis for effect level:

other: no effects

Target system / organ toxicity

Critical effects observed:

not specified