Dichloromethylbenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dichloromethylbenzene

EC Number:

249-854-8

EC Name:

Dichloromethylbenzene

Cas Number:

29797-40-8

Molecular formula:

C7H6Cl2

IUPAC Name:

(dichloromethyl)benzene

Test animals

Species:

rabbit

Strain:

New Zealand White

Test system

Type of coverage:

semiocclusive

Preparation of test site:

abraded

Vehicle:

unchanged (no vehicle)

Controls:

other: same animals served as control (one flanc: test substance, other flanc : water

Duration of treatment / exposure:

4 h

Observation period:

14 d

Number of animals:

3

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #1

Time point:

other: 1h, 24h, 48h, 72h, 7d, 14d

Score:

2.3

Max. score:

4

Reversibility:

not fully reversible within: 14 d

Results 2

Irritation parameter:

edema score

Basis:

animal #1

Time point:

other: 1 h, 24 h, 48 h, 72 h, 7 d, 14 d

Score:

1

Max. score:

4

Reversibility:

not fully reversible within: 14d

Results 3

Irritation parameter:

erythema score

Basis:

animal #2

Time point:

other: 1h, 24h, 48h, 72h, 7d, 14d

Score:

2

Max. score:

4

Reversibility:

not fully reversible within: 14d

Results 4

Irritation parameter:

edema score

Basis:

animal #2

Time point:

other: 1h, 24h, 48h, 72h, 7d, 14d

Score:

1

Max. score:

4

Reversibility:

not fully reversible within: 14d

Results 5

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

other: 1h, 24h, 48h, 72h, 7d, 14d

Score:

2.3

Max. score:

4

Reversibility:

not fully reversible within: 14d

Results 6

Irritation parameter:

edema score

Basis:

animal #3

Time point:

other: 1h, 24h, 48h, 72h, 7d, 14d

Score:

1

Max. score:

4

Reversibility:

not fully reversible within: 14d

Results 7

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

2.22

Max. score:

4

Reversibility:

not fully reversible within: 14 days

Results 8

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

1

Max. score:

4

Reversibility:

not fully reversible within: 14 days

Other effects:

In all animals from day 7 up to day 14 formation of scales was observed.

Any other information on results incl. tables

Results:

Animal No/ bw/ sex	DRAIZE-grade after
	1h		24h		48h		72h		7d		14d		Irritation scores
	Er	Ed	Er	Ed	Er	Ed	Er	Ed	Er	Ed	Er	Ed	Er	Ed
F59 (3.4 kg) female	1	1	2	1	3	1	2	1	1	0	0	0	2.3	1
G53 (3.0 kg) female	1	1	2	1	2	1	2	1	2	1	0	0	2.0	1
E51 (3.8 kg) female	1	1	2	1	3	1	2	1	1	1	0	0	2.3	1

Er = Erythema

Ed = Edema

In all animals from day 7 up to day 14 formation of scales was observed.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

Skin Irrit. 2 (not fully reversible within 14 days - formation of scales).

Executive summary:

In a skin irritation study according OECD Guideline 404, 3 female New Zealand White rabbits were treated with 500 µl of test substance to the abraded skin of one flank. The other flank served as control and was treated only with water. The test area was covered with a semiocclusive plaster. After 4 h the plasters were removed and the test areas were washed with water. Erythema and edema formation were evaluated after 24h, 48h, 72h, 7d and 14d. In all animals from day 7 up to day 14 formation of scales was observed. The test substance was found to be irritating.