Styrene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Dose range not specified, no details of signs of toxicity or histopathological findings were reported

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Toxicological studies of certain alkylated benzenes and benzene.

Author:

Wolf, M.A. et al.

Year:

1956

Bibliographic source:

AMA Arch Ind Health 14: 387-398

Reference 2

Reference Type:

secondary source

Title:

European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.

Author:

European Union

Year:

2007

Bibliographic source:

European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.

Materials and methods

Principles of method if other than guideline:

Different single doses of styrene were administered to Wistar rats by oral gavage.
Gross appearance of lungs, heart, liver, kidneys, spleen and testes was observed and organ weights determined.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Styrene

EC Number:

202-851-5

EC Name:

Styrene

Cas Number:

100-42-5

Molecular formula:

C8H8

IUPAC Name:

ethenylbenzene

Specific details on test material used for the study:

- Name of test material (as cited in study report): Styrene
- Physical state: liquid
- Analytical purity: > 99%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Biochemical Research Department, Dow Chemical Company from a stock obtained from the Wistar Institute
- Age at study initiation: young adult
- Weight at study initiation: 175 - 250 g
- Diet: modified Sherman stock diet: whole weat, freshly ground (55% by weight), dried whole milk (25% by weight), dried meat residue (or dried extracted liver) (12% by weight) dried brewer's yeast (5% by weight), Calcium carbonate (1% by weight), iodized table salt (2% by weight)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: undiluted or as olive-oil or corn-oil solution

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: < 7 cm3

Doses:

single doses, not specified: test item administrated undiluted or as olive-oil or corn-oil solution emulsified with a 5-10% aqueous solution of acacia (gum arabic)

No. of animals per sex per dose:

57

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: usually about 14 days
- Necropsy of survivors performed: no
- Other examinations performed: gross appearance of organs, organ weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 5 000 mg/kg bw

Gross pathology:

Slight liver changes and in some instances some kidney involvement of questionable significance were observed.

Any other information on results incl. tables

No further data were given.