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Diss Factsheets
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EC number: 202-851-5 | CAS number: 100-42-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- cell inhibition
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Preparation of bacteria stock cultures in agar vials. Stock cultures incubated for 24 h at 25°C. Continuous preparation of new stock cultures in a one week interval. For the preparation of the bacteria suspension bacteria are washed away by aid of sterile NaCl-solution and further diluted with NaCl-solution. Adjustment of extinction value to formacine-standard suspension. Preparation of different concentrations of the test solution. Preparation of final test solution: 80 mL test solution of respective concentrations, 5 mL stock solution I, 5 mL stock solution II and 10 mL bacteria suspension. 3 replicates of each concentration. Incubation of flaks for 16 hours at 25°C. At end of incubation period measurement of extinction of monochromatic radiation.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Neutralisation of test solution (addition of acid or base). Preparation of dilution series with sterile, destilled water: starting concentration 160 mL test solution, lower concenrations prepared by a spacing factor of 2. All concentrations for final test are filled up to a volume of 100 mL by adding 80 mL test solution, 5 mL of the stock solution I, 5 mL of the stock solution II and 10 mL of the bacteria suspension
- Controls: same (exception: for final test 10 mL NaCl-solution) - Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Test temperature:
- 25°C
- pH:
- 7.0
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks with cotton-filled plastic caps
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: glass, with headspace, 300 mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: Bacteria grown on agar culture medium and tested in test solution
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Toxic limit concentration mg/L [exticntion of monochromatic radiation]
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Duration:
- 16 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 72 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- other: starting inhibition effect on cell proliferation
- Executive summary:
Styrene was testet for possible toxicological effects in an test on Pseudomonas putida. The NOEC after 16h was found to be 72 mg/L.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 June 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Principles of method if other than guideline:
- Activated sludge, respiration inhibition test, 30 min
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on test solutions:
- - stock solution: for a concentration of 1200 mg/L: 0.3 g per vessel; for a concentration of 100 mg/L: 0.025 g
- synthetic medium: 8 mL per vessel
- inoculum: 50 mL per vessel
- dry substance: 1 g/L per vessel
- vessel volume: 250 mL - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20 - 25°C
- Nominal and measured concentrations:
- 100, 1200 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 1 - Reference substance (positive control):
- not specified
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Executive summary:
Styrene was testet for possible toxicological effects in an test on activated sludge of a predominantly domestic sewage. The EC50 after 30 min was found to be ca. 500 mg/L.
Referenceopen allclose all
Respiration rate (mg/l.h): 8 (at concentration of 1200 mg/L), 30 (at concentration of 100 mg/L).
Description of key information
Styrene was testet for possible toxicological effects in an acute test on activated sludge. The EC50 after 30 min was found to be 500 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 500 mg/L
Additional information
In short term respiration test conducted with activated sludge from municipal, non-adapted sewage treatment plant. resulted in an EC50 (30 min) and EC20 (30 min) of approx. 500 and 140 mg/l, respectively (BASF AG, 1988). The toxicity treshold (taken equivalent to NOEC) for Pseudomonas putida after an exposure time of 16 hours was 72 mg/l (Bringmann & Kühn, 1977). Other cell multiplication tests conducted with bacteria and protozoa (Bringmann & Kühn, 1973,1978 ,1980 and 1982, Qureshi, 1982, Andreeva, 2019, Mosafa, 2016) indicate similar (or lower) sensitivity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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