Registration Dossier
Registration Dossier
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EC number: 202-851-5 | CAS number: 100-42-5
Immunotoxicity
Administrative data
- Endpoint:
- immunotoxicity
- Remarks:
- other: modulation of in vitro cytokine production
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficient for assessment; in vitro immune-modulatory effect on cytokines by SO
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- TH1-directed modulation of in vitro cytokine production in human peripheral blood mononuclear cells by styrene-7,8-oxide
- Author:
- Merker, G.H. et al.
- Year:
- 2 006
- Bibliographic source:
- Toxicology Letters 160: 105-111
- Reference Type:
- review article or handbook
- Title:
- European risk assessment report, Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007.
- Author:
- European Union
- Year:
- 2 007
- Bibliographic source:
- Styrene CAS No. 100-42-5, EINECS No. 202-851-5, Draft for submission to SCHER, November 2007
Materials and methods
- Principles of method if other than guideline:
- The modulation of the immune system by styrene-7,8-oxide in vitro was investigated. Human peripheral blood mononuclear cells were incubated with styrene-7,8-oxide, either as (S)-enantiomer, (R)-enantiomer, or racemic styrene-7,8-oxide. Subsequently, the secretion of TH1-cytokines IFNγ and IL-12 as well as TH2-cytokines IL-4 and IL-5 were measured by ELISA.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Styrene
- EC Number:
- 202-851-5
- EC Name:
- Styrene
- Cas Number:
- 100-42-5
- Molecular formula:
- C8H8
- IUPAC Name:
- ethenylbenzene
Constituent 1
- Specific details on test material used for the study:
- The substance tested is a metabolite of styrene.
Test animals
- Species:
- other: in vitro: human peripheral blood mononuclear cells
- Strain:
- other: not applicable
- Sex:
- not specified
Administration / exposure
- Route of administration:
- other: not applicable
- Vehicle:
- other: acetonitrile
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- not applicable
- Frequency of treatment:
- no data
Doses / concentrations
- Remarks:
- Doses / Concentrations:
different concentrations between 0.1344 and 262,750 ng/ml
Basis:
no data
- No. of animals per sex per dose:
- not applicable
- Control animals:
- other: in vitro: solvent control
Results and discussion
Any other information on results incl. tables
Results revealed a stimulation of cytokine secretion with emphasis on TH1 -cytokines IFNγ and IL-12. The stimulating effects were elicited at concentrations of styrene-7,8-oxide comparable to what would be encountered at industrial workplaces where styrene is processed. Styrene-7,8-oxide exhibits immunomodulatin capacities, which can be of clinical relevance for individuals with high styrene exposure.
Applicant's summary and conclusion
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