Cyanoacetic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

no data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study. No test with a strain detecting oxidizing / cross-linking activity (e.g. Salmonella typhimurium TA102 / Escherichia coli WP2 uvrA) included.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

Version / remarks:

cited as EEC Directive 84/449, B14.

Deviations:

no

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Cyanoacetic acid

EC Number:

206-743-9

EC Name:

Cyanoacetic acid

Cas Number:

372-09-8

Molecular formula:

C3H3NO2

IUPAC Name:

cyanoacetic acid

Details on test material:

- Name of test material (as cited in study report): Cyanessigsäure, techn. krist. (cyanoacetic acid)
- Physical state: solid (white to light yellow-green, crystalline substance)
- Analytical purity: 98.6%
- Lot/batch No.: 08511
- Storage condition of test material: at room temperature
No further data.

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

Aroclor 1254induced rat liver S-9

Test concentrations with justification for top dose:

4 - 5000 µg/plate; for details, see freetext below

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Negative solvent / vehicle controls:

yes

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene, methyl-N'-nitro-N-nitrosoguanidine, 4-nitro-o-phenylendiamine, 9-aminoacridine chloride monohydrate

Remarks:

Positive control substance depending on the tester strain and activation condition

Details on test system and experimental conditions:

METHOD OF APPLICATION: in agar (plate incorporation); preincubation

NUMBER OF REPLICATIONS: 3

DETERMINATION OF CYTOTOXICITY
- Method: reduced his- background growth, decrease in the number of his+ revertants

Evaluation criteria:

In general, a substance to be characterized as positive in the Ames test has to fulfill the following requirements:
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the results.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

>= 1000 µg/plate

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Mutagenicity

An increase in the number of his+ revertants was not observed both in the standard plate test and in the preincubation test either without S-9 mix or after the addition of a metabolizing system.

Toxicity

A bacteriotoxic effect (reduced his- background growth, decrease in the number of his+ revertants) was observed, depending on the strain and test conditions at doses >= 1000 µg/plate.

Solubility

Complete solubility of the test substance in distilled water at all concentrations tested.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative