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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP, guideline study however the purity of the test substance was < 80 %

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyanoacetic acid
EC Number:
206-743-9
EC Name:
Cyanoacetic acid
Cas Number:
372-09-8
Molecular formula:
C3H3NO2
IUPAC Name:
cyanoacetic acid
Details on test material:
- Name of test material (as cited in study report): Cyanessigsaure roh 70%

- Lot/batch No.: 001V28AX10
- Physical state: liquid
- Analytical purity: 71.4 g/100g
- Composition of test material, percentage of components: 71.4 % Cynoacetic acid, 28.6 % water

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from laboratory wastewater plants treating municipal sewage.
- Concentration of sludge: 30 mg/I dry substance
Duration of test (contact time):
17 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
66 mg/L
Based on:
test mat.
Details on study design:
TEST CONDITIONS
- Composition of medium: 5 ml/vessel inoculum, 13 ml/vessel inorganic medium, 68 ml/vessel stock solution, filled up to a total volume of 1000 ml/vessel with dionised water
- pH: 7.4


TEST SYSTEM
- Number of culture flasks/concentration: 2



CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
90 - 100
Sampling time:
17 d

BOD5 / COD results

Results with reference substance:
90 - 100 % after 14 days

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable