Registration Dossier
Registration Dossier
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EC number: 206-743-9 | CAS number: 372-09-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, however the purity of the test substance was < 80 %
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cyanoacetic acid
- EC Number:
- 206-743-9
- EC Name:
- Cyanoacetic acid
- Cas Number:
- 372-09-8
- Molecular formula:
- C3H3NO2
- IUPAC Name:
- cyanoacetic acid
- Details on test material:
- - Name of test material (as cited in study report): Cyanoacetic acid roh 70%
- Physical state: liquid
- Analytical purity: 71.4 %
- Lot/batch No.: 001V28AX10
- Composition of test material, percentage of components: 71.4 %, 21.5 % water
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in deionized water for about 20 min at approximately 20 +/- 2 °C. The pH-value of the stock solution (pH = 3.0) was adjusted to pH = 7.5. The nominal concentration of the stock solution was 125.2 mg/l. Serial dilutions of this stock solution were then prepared in order to obtain the desired test concentrations.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: CHODAT SAG 86.81
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23 +/- 2 °C
- pH:
- 8.0 - 9.2
- Nominal and measured concentrations:
- Nominal test concentrations (mg/l):
0.137,
0.412,
1.23,
3.70,
11.1,
33.3,
100.0,
control
Measured test concentrations (mg/l)
after 0 h:
control: <0.5
100 mg/l: 99.5
11.1 mg/l: 11.5
1.23 mg/l: 1.27
after 72 h:
control: <0.5
100 mg/l: 101.2
11.1 mg/l: 11.1
1.23 mg/l: 1.27 - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable siliconsponge caps
- Fill volume: 100 ml
- Initial cells density: 10000
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes, according to OECD guidelines.
OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: artificial light, type repluniversal white (OSRAM L 25), about 60 - 120 microE/(m2*s) at a wave length of 400 - 700 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber (Neubauer improved)
- Chlorophyll measurement: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.23 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.85 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 98.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- At the test concentrations of 100 mg/l to 1.23 mg/l clumps of algae were observed in the inoculated vessels. After shaking the clumps were disturbed and the algae cells were uniformly distributed
Applicant's summary and conclusion
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
