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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Remarks:
The acute earthworm toxicity study was conducted solely to comply with a non-EU national registration requirement, and has been provided here in accordance with REACH, Article 22(1)e.
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
From 24 June 2014 to 25 August 2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP.
Qualifier:
according to
Guideline:
other: MEP, P.R. China the guidelines for the Testing of Chemical No.207 "Earthworm, acute Toxicity Test"
Version / remarks:
Version 2 / 2013
Deviations:
no
Qualifier:
according to
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Version / remarks:
1984
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)
Version / remarks:
EPA 712-C-96-167 / April 1996
Deviations:
no
Qualifier:
according to
Guideline:
other: SEPA, the guidelines for Hazard Evaluation of New Chemical Substances, Environmental Protection agency of P.R.C. (HJ/T 154-2004)
Version / remarks:
2004
Deviations:
no
GLP compliance:
no
Remarks:
The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP.
Analytical monitoring:
no
Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil (if used): the test item was weight and mixed with fine ground quartz. According to the soil mixture content, 2822.5 g wet artificial soil (dry weight is 2350 g) was weighed and mixed the above mixture of test item. 300 mL distilled water was added to adjust the moisture content to ca. 35% of dry weight.
- Method of application to soil surface (if used): after fully mixing by blender for 40 minutes, four 750 g (753.2 - 753.9 g) of the test medium were weighed and put into its corresponding labeled test container.
- Controls: yes, prepared under identical procedures but did not contain the test item.
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: Eisenia Foetida
- Source: Chenggong Earthworm raising Farm, Dongli district, Tianjing.
- Age at test initiation (mean and range, SD): More than two months old
- Weight at test initiation (mean and range, SD): 300.9 to 489.5 mg

ACCLIMATION
- Acclimation period: 24 hours
- Health during acclimation (any mortality observed): no mortality observed
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
20.0 - 21.7%
Moisture:
67 to 86%
Details on test conditions:
TEST SYSTEM
- Test container (material, size): Height 19cm X Lenght: 12cm X width: 9cm
- Amount of soil or substrate: 2350 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
Artificial soil:
a) sphagnum peat moss 10%
b) Kaolin clay 20%
c) Quartz sand 68%
d) Calcuim carbonate 2%


OTHER TEST CONDITIONS
- Photoperiod: light was kept continuous
- Light intensity: 418 to 454 Lux


TEST CONCENTRATIONS
The results of the preliminary test showed that the LC50 was greater than 1000 mg/kg dry soil weight. The definitive test was conducted with a limit test with a concentration of 1000 mg/kg dry soil weight.
Nominal and measured concentrations:
1000 mg/kg dry soil weight
Reference substance (positive control):
no
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: no mortality
- Total mass of adults at beginning of test: 0.3859 g ±0.0509
- Total mass of adults at beginning of test: 0.2665 g ±0.0468

During the exposure period, no toxic symptoms was observed.

Validity criteria fulfilled:
yes
Conclusions:
The 14-day LC50 is greater than 1000 mg/kg dry soil weight.
Executive summary:

The study was performed to assess the acute toxicity of CA4920 to the earthworm.

Based on the results of preliminary test, the definitive study was conducted with a concentration of 1000 mg/kg dry soild weight. a concurrent control group was conducted in parallel. For the test and control groups, 4 replicates were used with 10 worms in each replicate. Artificial soil was used. The test duration was 14 days. After 7 days and 14 days, toxic symptoms were recorded and the mortality was assessed.

Description of key information

The study was performed to assess the acute toxicity of CA4920 to the earthworm.

Based on the results of preliminary test, the definitive study was conducted with a concentration of 1000 mg/kg dry soild weight. a concurrent control group was conducted in parallel. For the test and control groups, 4 replicates were used with 10 worms in each replicate. Artificial soil was used. The test duration was 14 days. After 7 days and 14 days, toxic symptoms were recorded and the mortality was assessed.

The 14 -day LC50 was found to be greater than 1000 mg/kg dry soil weight.

Key value for chemical safety assessment

Additional information