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Toxicity to terrestrial plants

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Reference
Endpoint:
toxicity to terrestrial plants: short-term
Remarks:
The seed germination / root elongation toxicity study was conducted solely to comply with a non-EU national registration requirement, and has been provided here in accordance with REACH, Article 22(1)e.
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From 16 May 2014 to 28 August 2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP.
Qualifier:
according to
Guideline:
other: MEP, P.R. China the Guidelines for Testing of Chemicals No. 299 "Seed Germination/Root elongation Toxicity Test"
Version / remarks:
Version 2 / 2013
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 850.4200 (Seed Germination/Root Elongation Toxicity Test)
Version / remarks:
EPA 712-C-96-154 / 1996
Deviations:
no
GLP compliance:
no
Remarks:
The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP.
Analytical monitoring:
yes
Details on sampling:
- Concentrations: Control and 10 mg/L test solutions
- Sampling method: During the test, with the test samples, procedural recovery sample with concentration of 1.0 mg/L was analysed. the recovery samples were prepared by direct addition of the stock solution to test water.
- Sample storage conditions before analysis: Not reported
Vehicle:
yes
Remarks:
water
Details on preparation and application of test substrate:
- Method of application to filter paper (if used): Matrix of qualitative filter paper (diameter 18cm) in five layer.

Exposure solution was prepared by adding a measured weight of 0.03007 g of test item to a glass bottle and then 1 liter of water was added in. After mixing with ultrasonication (12 min) the bottle was filled with water to get a final solution of 3 liter and put onto the magnetic stirrer and stirred for about 22 hours. The solution was then filtered by 0.45 µm filter membrane and used for the test.
Species:
other: Glycine max
Details on test organisms:
- Common name: Soybean
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times and soaked for about 2 hours before used.
Species:
Lycopersicon esculentum
Details on test organisms:
- Common name: Tomato
- Prior seed treatment/sterilization: : the seed was rinsed with distilled water for 5 times before used.
Species:
Zea mays
Details on test organisms:
- Common name: Corn
- Prior seed treatment/sterilization: : the seed was rinsed with distilled water for 5 times and soaked for about 4 hours before used.
Species:
Cucumis sativus
Details on test organisms:
- Common name: Cucumber
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times before used.
Species:
Oryza sativa
Details on test organisms:
- Common name: Rice
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times and soaked for about 24 hours before used.
Species:
Lolium perenne
Details on test organisms:
- Common name: Ryegrass
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times before used.
Species:
Triticum aestivum
Details on test organisms:
- Common name: Common Wheat
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times before used.
Species:
other: Medicago Sativa
Details on test organisms:
- Common name: Alfalfa
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times before used.
Species:
other: Vignaradiate
Details on test organisms:
- Common name: Mung bean
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times and soaked for about 24 hours before used.
Species:
Brassica rapa
Details on test organisms:
- Common name: Canola
- Prior seed treatment/sterilization: the seed was rinsed with distilled water for 5 times before used.
Test type:
seed germination/root elongation toxicity test
Study type:
laboratory study
Substrate type:
filter paper
Limit test:
yes
Remarks:
The exposure period ended when at least 65 percent of the control seed had germinated and developed roots that are 20 mm long.
Test temperature:
25 ± 1 °C
pH:
7.49 - 7.63
Moisture:
not specified
Details on test conditions:
TEST SYSTEM

- Test container (type, material, size): Petri dishes
- Method of seeding: The seed was positioned on five qualitatives filter paper
- No. of seeds per container: 10
- No. of replicates per treatment group: 3
- No. of replicates per control: 3


GROWTH CONDITIONS
- Photoperiod: Complete darkness


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Number of germinated seeds were counted and root lengths were measured at the end of the study. The test period ended when at least 65 % of the control seed had germinated and developed roots as least 20 mm long.



Nominal and measured concentrations:
Nominal concentrations: 10 mg/L
Measured concentrations: 1.399 mg/L
Reference substance (positive control):
no
Key result
Species:
Zea mays
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
Cucumis sativus
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.339 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
Lycopersicon esculentum
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
Lolium perenne
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
Triticum aestivum
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
other: Medicago Sativa
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
other: Vignaradiate
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
Oryza sativa
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
other: Glycine max
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
other: Brassica rapa
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
germination
Key result
Species:
Zea mays
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
Cucumis sativus
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
Lycopersicon esculentum
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
Lolium perenne
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
Triticum aestivum
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
other: Medicago sativa
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
other: Vignaradiate
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
Oryza sativa
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
other: Glycine max
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Key result
Species:
Brassica rapa
Duration:
5 d
Dose descriptor:
EC50
Effect conc.:
> 1.399 other: mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
other: root elongation
Details on results:
SEED GERMINATION
- Percent seed germination: At the end of the test, over 65 % of the control seeds germinated
- Root length: At the end of the test, control roots were longer than 20 mm
Reported statistics and error estimates:
Two tailed T-tests were conducted for each test species to determine wether significant inhibited/enhanced root growth (p<0.05) appeared in the test group compared to the control.
Validity criteria fulfilled:
yes
Remarks:
> 65 % of the control seed germinated at the end of the test
Conclusions:
The EC50 for seed germination and root elongation of the test material to Lolium perenne (Ryegrass), Triticum Aestivum (Common wheat), Medicago sativa (Alfalfa), Vignaradiate (Mung bean), Oryza sativa L. (rice), Cucumis sativus (cucumber), Zea mays (corn), Lycopersicon esculentum (tomato), Glycine max (soybean) and brassica rapa (Canola) was greater than 1.399 mg7L (initial measured concentration).
Executive summary:

The effect of the test material on seed germination and root elongation of c Lolium perenne (Ryegrass), Triticum Aestivum (Common wheat), Medicago sativa (Alfalfa), Vignaradiate (Mung bean), Oryza sativa L. (rice), Cucumis sativus (cucumber), Zea mays (corn), Lycopersicon escule ntum (tomato), Glycine max (soybean) and brassica rapa (Canola) was determined. Seedlings were exposed to initial measured concentrations of 1.399 mg/L, alongside a blank control. Each replicate contained 10 seeds and 3 replicate was tested for each treatment. The EC50 for seed germination and root elongation of the test material was greater than 1.399 mg/L for all plant species tested. This study is considered to be reliable with restrictions (Klimisch 2) as it was conducted according to SEPA HJ/T 153-2004.

Description of key information

The effect of the test material on seed germination and root elongation of c Lolium perenne (Ryegrass), Triticum Aestivum (Common wheat), Medicago sativa (Alfalfa), Vignaradiate (Mung bean), Oryza sativa L. (rice), Cucumis sativus (cucumber), Zea mays (corn), Lycopersicon escule ntum (tomato), Glycine max (soybean) and brassica rapa (Canola) was determined. Seedlings were exposed to initial measured concentrations of 1.399 mg/L, alongside a blank control. Each replicate contained 10 seeds and 3 replicate was tested for each treatment. The EC50 for seed germination and root elongation of the test material was greater than 1.399 mg/L for all plant species tested.

Key value for chemical safety assessment

Additional information