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Long-term toxicity to aquatic invertebrates

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Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Remarks:
The Daphnia Magna reproduction toxicity study was conducted solely to comply with a non-EU national registration requirement, and has been provided here in accordance with REACH, Article 22(1)e.
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
From 01 April 2014 to 27 June 2014
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP.
Qualifier:
according to
Guideline:
other: MEP, P.R. China, the Guidelines for Testing of Chemicals No. 211 "Daphnia magna Reproduction Test"
Version / remarks:
Version 2 / 2013
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Version / remarks:
02 October 2012
GLP compliance:
no
Remarks:
The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP.
Analytical monitoring:
yes
Details on sampling:
- Concentrations: 0.625, 1.25, 2.5, 5.0 and 10 mg/L were analysed
- Sampling method: Three samples were collected during the definitive test fro analysis (freshly prepared media on 0, 2, 9 and 12 day after exposure, and expired media on 2, 9, 12 and 21 day after exposure.
Vehicle:
yes
Remarks:
water
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Concentration of vehicle in test medium (stock solution): 10 mg/L
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Applying Chemsitry department Pesticide Environmental Evaluation Lab of China agricultural University.
- Age of parental stock: Less than 24 hours old
- Feeding during test: Yes
- Food type: living algal cells
- Amount: 0.1 to 0.2 mg Cells/Daphnia/ day
- Frequency: daily


Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Hardness:
140-250 mg/L (as caCO3)
Test temperature:
20 ± 1°C
pH:
6-9
Dissolved oxygen:
Above 3 mg/L
Salinity:
not applicable
Nominal and measured concentrations:
Nominal: 0.0625, 1.25, 2.5, 5.0 and 10 mg/L
Measured: 0.062, 0.130, 0.260, 0.523 and 1.06 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL glass beakers
- Type (delete if not applicable): loosely covered
- Aeration: 24 hours before usage
- Renewal rate of test solution (frequency/flow rate): three times a week
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: demineralized water treated with special softening technics.


OTHER TEST CONDITIONS
- Photoperiod: 16hr light / 8 hr dark
- Light intensity:1000 - 1500 lux

EFFECT PARAMETERS MEASURED :
-pH value, dissolved oxygen, water hardeness, light condition and temperature

TEST CONCENTRATIONS
- Test concentrations: 0.0625, 1.25, 2.5, 5.5 and 10 mg/L
Reference substance (positive control):
no
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.062 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks:
first brood time
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.26 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
total living offspring for each parent
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.26 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
morphology
Remarks:
Body length of parent
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.26 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Parental mortality
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
1.06 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
Total dead offspring for each parent
Details on results:
- Behavioural abnormalities:
- Observations on body length and weight: significant difference (p<0.01) between control and test concentration of 5.0 mg/L
- Mortality of control: one parent animal died accidentally due to handling error in replicate 10
Validity criteria fulfilled:
yes
Conclusions:
The NOEC for the first brood time was found to be 0.062 mg/L.
Executive summary:

A reproduction test of the test item was conducted with Daphnia magna over a 21 -day exposure period. The definitive test was conducted with the concentrations of 0.625, 1.25, 2.5, 5 and 10 mg/L (with mean measured concentrations of 0.062, 0.130, 0.260, 0.523 and 1.06 mg/L, concurrently with a blank control group. 10 animals individually held at each test concentration and controls were used.

The test was performed with a semi-static water renewal system.

Total number of living juveniles of each parent, body lenght, parental mortality and total number of dead offspring were calculated. The concentrations were determined in samples of test media by HPLC. The resulst showed that the mean measured concentration of the test item were in the range of 95.4% - 97.1% of the initial measured concnetration.

Description of key information

A reproduction test of the test item was conducted with Daphnia magna over a 21-day exposure period. The definitive test was conducted with the concentrations of 0.625, 1.25, 2.5, 5 and 10 mg/L (with mean measured concentrations of 0.062, 0.130, 0.260, 0.523 and 1.06 mg/L, concurrently with a blank control group. 10 animals individually held at each test concentration and controls were used.

The test was performed with a semi-static water renewal system.

Total number of living juveniles of each parent, body lenght, parental mortality and total number of dead offspring were calculated. The concentrations were determined in samples of test media by HPLC. The resulst showed that the mean measured concentration of the test item were in the range of 95.4% - 97.1% of the initial measured concnetration.

The NOEC for the first brood time was found to be 0.062 mg/L.

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.062 mg/L

Additional information