3-chloro-p-toluidine

Genetic toxicity: in vivo

Administrative data

Endpoint:

genetic toxicity in vivo, other

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: New test system without generally acceptance.

Data source

Reference

Reference Type:

publication

Title:

An in vivo-in vitro replicative DNA synthesis (RDS) test using rat hepatocytes as an early prediction assay for nongenotoxic hepatocarcinogens~ screening of 22 known positives and 25 noncarcinogens

Author:

UnoY, Takasawa H, Miyagawa M, Inoue Y, Murata T and Yoshikawa K

Year:

1994

Bibliographic source:

Mutation Research, 320 (1994) 189-205

Materials and methods

Principles of method if other than guideline:

To evaluate the applicability of an in vivo-in vitro replicative DNA synthesis (RDS) test using rat hepatocytes, we conducted the RDS test with 22 nongenotoxic (Ames-negative) hepatocarcinogens and 25 noncarcinogens (including 3-chloro-p-toluidine, CAS 95-74-9) under standardized conditions and judgement criteria.

GLP compliance:

no

Type of assay:

other: n vivo-in vitro replicative DNA synthesis (RDS) test

Test material

Test material information

Constituent 1

Reference substance name:

3-chloro-p-toluidine

EC Number:

202-446-3

EC Name:

3-chloro-p-toluidine

Cas Number:

95-74-9

Molecular formula:

C7H8ClN

IUPAC Name:

3-chloro-4-methylaniline

Test animals

Species:

rat

Applicant's summary and conclusion

Executive summary:

3-chloro-p-toluidine clearly stimulated RDS at 24 or 39 h after treatment. 3-chloro-p-toluidine is regarded as a noncarcinogen. Therefore the test system is not suitable for mutagenicity testing of 3-chloro-p-toluidine.