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EC number: 266-582-5
CAS number: 67124-09-8
Static freshwater test with Daphnia magna resulted in EC50 of 0.58 mg/L and a NOELR of 0.32 mg/L
experimental investigations are available concerning the acute toxicity
to aquatic invertebrates of the test substance was investigated
according to OECD Guideline 202 (EU Method C.2). The
studies were conducted under certificated GLP compliance and Daphnia
used as test organisms, which is a freshwater invertebrate
representative of a wide variety of natural habitats, and can therefore
be considered as an important non-target organism in freshwater
ecosystems. In-house laboratory cultures of the test animals were used,
whereby young daphnids, less than 24 h old were used for testing. These
daphnids were not individually identified and received no food.
concentration to be used in the definitive test of the key study was
determined by preliminary range-finding tests (Goodband and Mullee,
2011). Based on these range-finding studies, concentrations of 0.1,
0.18, 0.32, 0.56 and 1.0 mg/L nominal loading rate WAFs were tested. Two
duplicates were prepared for each test group, two replicates for the
control. No aeration was performed and no evidence of undissolved
substance was observed, since Microscopic inspection of the WAFs showed
no micro-dispersions or undissolved test item to be present. The
solutions were not renewed during the exposure period. The vessels were
covered to reduce evaporation and maintained at 21.0 - 22.0 °C in a
temperature controlled room with a photoperiod of 16 h light and 8 h
darkness with 20 min dawn and dusk transition periods for a test
duration of 48 hours. A positive control used potassium dichromate as
the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2
mg/L was conducted. The EC50 value was determined to be 0.58 mg/L
loading rate WAF with 95 % confidence limits of 0.51 - 0 .66 mg/L. The
corresponding No Observed Effect Loading Rate (NOELR) is 0.32 mg/L. Gas
chromatography (GC) was the analytical method of choice.
based on range-finding
studies, concentrations of 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100
mg/L nominal loading rate WAFs were used in the supporting study
(Sewell, 2004). Other test conditions were the same as reported in the
key information. In this case, the
EC50 value was determined to be 1.3 mg/L loading rate WAF with 95 %
confidence limits of 1.0 - 1.8 mg/L. The corresponding No Observed
Effect Loading Rate (NOELR) is 1.0 mg/L. No analytical method was used.
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