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EC number: 266-582-5
CAS number: 67124-09-8
Scenedesmus subspicatus (OECD 201): EL50(96h) > 100 mg/L loading rate WAF, NOELR: 100 mg/L loading rate WAF
The growth inhibition effect of the test
substance on the green algae species Desmodesmus subspicatus (strain:
CCAP 276/20, reported as Scenedesmus subspicatus) was
investigated according to OECD guideline 201(Mead, 2004). Following a
preliminary range-finding test, the test organisms were exposed to a
Water Accomodated Fraction (WAF) of the test material at a single
nominal loading rate of 100 mg/L (six replicate flasks) for 96 hours
under constant illumination (approximately 4000 Lux) and shaking. The
temperature was held constant at 24 ±
1 °C. Samples of the algal population were collected daily and cell
concentrations determined for each control and treatment group using a
Multisizer Particle Counter. As results an Effective Loading rate, EL50
greater than 100 mg/L loading rate WAF and the corresponding No Observed
Effect Loading Rate of 100 mg/L is reported. It was considered
unnecessary and unrealistic to test a loading rate in excess of 100 mg/L.
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