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EC number: 266-582-5
CAS number: 67124-09-8
Daphnia magna reproduction test (OECD 211): Immobilisation: EL50(21d): 0.75 mg/L, NOELR: 0.32 mg/L / Reproduction: EL50(21d): 0.50 mg/L, LOELR: 0.56 mg/L
The long-term toxicity to invertebrates of
the test substance to Daphnia magna was investigated according to OECD
Guidelines for Testing of Chemicals No 211 (1998) "Daphnia magna,
Reproduction Test" (Wetton, 2003). Based on the results of peliminary
range-finding tests, the invertebrates were exposed (10 replicates of a
single daphnid per group) to filtered Water Accommodated Fractions
(WAFs) of the test material over a range of loading rates of 0.10, 0.18,
0.32, 0.56 and 1.0 mg/L for a period of 21 days. The test solutions were
renewed 3 times per week. The numbers of live and dead adult Daphnia
and young daphnids (live and dead) were determined daily. The Daphnia
were fed daily with an algal suspension (Chlorella sp.),
whereby the level was approximately 0.1 - 0.2 mg carbon/daphnid/day,
dependent on the age and size of the animal. Adult Daphnia unable
to swim for approximately 15 seconds after gentle agitation (ie.
immobile), were considered to be dead. An immobilisation criterion for
the young daphnids was considered to be inappropriate due to the large
numbers of off-spring produced in the flasks.The 14 and 21-Day
EL50 (immobilisation) value, based on nominal loading rates, for the
parental Daphnia generation (P1) was calculated to be 0.75 mg/L loading
rate WAF with 95 % confidence limits of 0.56 – 1.0 mg/L loading rate
WAF. The 21-Day EL50 (reproduction) value based on nominal loading rates
was calculated to be 0.50 mg/L loading rate WAF with 95 % confidence
limits of 0.39 – 0.63 mg/L loading rate WAF. The "Lowest Observed Effect
Loading Rate" was considered to be 0.56 mg/L on the basis that at this
test concentration significantly fewer live young per adult (P < 0.05)
were produced when compared to the control. The "No Observed Effect
Loading Rate " was considered to be 0.32 mg/L on the basis that at this
test concentration there were no mortalities (immobilisation) observed
in the parental generation (P1) and that there were no significant
differences (P ≥
0.05) between the control and the 0.32 mg/L loading rate WAF test group
in terms of numbers of live young produced per adult by Day 21.
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