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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Skin sensitisation

Currently viewing:

Administrative data

Endpoint:
skin sensitisation: in vivo (non-LLNA)
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Sensitization of guinea-pig skin to phenylhydrazine
Author:
Jadassohn
Year:
1930
Bibliographic source:
Klinische Wochenschrift, 9:551; cited in WHO 2000, Phenylhydrazine. CICAD 19, Wissenschaftliche Verlagsgesellschaft mbH
Reference Type:
secondary source
Title:
Concise International Assessment Document 19: Phenylhydrazine
Author:
WHO
Year:
2000
Bibliographic source:
Wissenschaftliche Verlagsgesellschaft mbH, Stuttgart, ISBN 92 4 153019 7; ISSN 1020-6167

Materials and methods

Principles of method if other than guideline:
The skin of guinea pigs were painted with phenylhydrazine. After 2 - 3 weeks the not pretreated skin was painted with a 10 % alcoholic solution of phenylhydrazine.
GLP compliance:
no
Type of study:
skin painting test

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): "Phenylhydrazin", phenylhydrazine

In vivo test system

Test animals

Species:
guinea pig
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
no data

Study design: in vivo (non-LLNA)

Inductionopen allclose all
Route:
epicutaneous, open
Vehicle:
other: alcohol
Concentration / amount:
10 %
Challengeopen allclose all
Route:
epicutaneous, open
Vehicle:
other: alcohol
Concentration / amount:
10 %
No. of animals per dose:
no data
Details on study design:
RANGE FINDING TESTS: no data

MAIN STUDY
A. INDUCTION EXPOSURE
- two to three weeks before the challenge exposure, the skin was treated with undiluted phenylhydrazine.

B. CHALLENGE EXPOSURE
- 10 % solution phenylhydrazine in alcohol was painted on an area other than the pretreated skin site.
Challenge controls:
no data
Positive control substance(s):
no

Results and discussion

Positive control results:
no data

Any other information on results incl. tables

A very intense erythema and swelling, followed by scaling and encrustation, were consistently seen within 24 hours. This response to 10% phenylhydrazine was more severe than that described in animals that had not been pretreated.

Applicant's summary and conclusion