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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline Study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- TS: CPH, BAS 500F-Stufe 100
- Purity >99 %
- Inpurity: toluene <0.5 %

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
- Preparation of stock solution: The test was performed with an aqueous extract (eluate) of the test substance.
- The test substance was stirred in M4 medium for about 20 hours at 20 +/- 2°C.
- Undissolved test substance was removed by centrifugation (approximately 20 min at about 17700 g).
- The nominal concentration of the eluate was 100 mg/L.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Daphnia magna Straus was originally supplied from "Institut National de Recherche Chimique appliquee, France", and has been breeded from 1978 in the Laboratories of BASF.
- 2 - 24 hours old (starting with the 3rd breed of parent animals)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20.3 - 20.9 °C
pH:
7.5 to 8.1
Dissolved oxygen:
4.9 to 8.6 mg/L
Nominal and measured concentrations:
The analytical recovery rate was after 0/48 h in all analyzed test solutions below the detection limit (< 1 mg/L). The effective concentrations mentioned here are based therefore on the nominal concentrations of the test substance. Nominal concentrations in mg/L: control, 100, 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78 and 0.39
Details on test conditions:
Test conditions:
- Dilution water chemistry: A synthetic fresh water was used for culture and test purposes.
- This water was prepared on the basis of an ultrapure, demineralized water with a conductivity <0.05 uS/cm.
- For the composition of this M4 medium see ISO 10706.
- Properties of this medium:
Total hardness: 2.20 - 3.20 mmol/L
Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L
Molar ratio Ca:Mg about 4:1
pH value: 7.5 - 8.5
Conductivity: 550 - 650 uS/cm

After preparation the M4 medium is aerated tor approximately 24 h until saturation with oxygen is reached.
- Exposure vessels: test tubes (glass) with flat bottom (nominal volume 20 ml)
- Light conditions: Artificial light, type warm white, day:night rhythm = 16:8 h
- Number of Replicates: 4
- Individuals /replicate: 5

Statistics:
- EC50 values have been calculated with the moving average method.

Measurements:
- pH values and dissolved oxygen: After Oh in an additional replicate without daphnids and after 48h in replicate 1 of each tested concentration.
- Mobile daphnids: Visually after 0, 3, 6, 24 and 48 h


Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
1.56 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.68 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

effect values (related to nominal concentration)
-----------------------------------------------------------------------
time EC0 EC50 confidence limits EC100
(h) (mg/L) (mg/L) 95 % (mg/L) (mg/L)
3 12,5 26,8 23,0 - 31,2 50
6 12,5 17,7 n.d - n.d. 25
24 3,13 7,64 6,44 - 9,07 12,5
48 1,56 2,68 2,17 - 3,31 6,25


Mobile daphnids after x hours:
Concentration [nominal] 3h 6h 24h 48h
Control (0 mg/L) 20 20 20 20
100 mg/l        0 0 0 0
50 mg/l                0 0 0 0
25 mg/l               12 0 0 0
12.5 mg/l        20 20 0 0
6.25 mg/l         20 20 16 0
3.13 mg/l        20 20 19 7
1.56 mg/l        20 20 19 18
0.78 mg/l        20 20 19 19
0.39 mg/l        20 20 20 18              

Applicant's summary and conclusion