Chromate(2-), [2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-[(4,5-dihydro-3-methyl-5-oxo-1-phenyl-1H-pyrazol-4-yl)azo]-2-hydroxy-5-nitrobenzenesulfonato(3-)]-, disodium

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.5 (Degradation: Biochemical Oxygen Demand)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT20010/C
Batch-Nr: 149
Purity ca: 70 %
Appearance: solid
Solubility: >1 g/1 miscible [in water]
Storage: room temperature
Expiration date: 12/99

Oxygen conditions:

aerobic

Inoculum or test system:

other: Seeding water of a communal sewage treatment plant Pro Rheno Basel collected on June 28,1995

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): communal sewage treatment plant Pro Rheno Basel collected on June 28,1995

Duration of test (contact time):

5 d

Initial conc.:

6 - 8 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature:: 20 ± 1

Reference substance:

other: D(*) - Glucose (Merck Nr. 8337) / L-Glutamic acid (Merck Nr. 291)

Remarks on result:

not measured/tested

Parameter:

BOD5

Value:

0 mg O2/g test mat.

Results with reference substance:

The BOD5 of Glucose/Glutamic acid solution is 202 mg O2/L

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The BOD5 of FAT 20010/C was found to be 0 mg O2/g

Executive summary:

The biochemical oxygen demand of FAT 20010/C was determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of micro-organisms. The test was carried out according to ISO 5815 Second Edition 1989-08-01 (E) corresponding to 92 / 69 / EEC C.5. The test substance was tested in concentrations from about 6 to 800 mg/I. The reference substance D(+)-Glucose / L-Glutamic acid was tested in a concentration of 20 ml/l. Based on the findings of the study, the BOD5 of FAT 20010/C is 0 mg O2/g. The BOD5 of Glucose/Glutamic acid solution is 202 mg O2/I. In another experiment, the COD of FAT 20010/C was found to be 1100 mg O2/g. The BOD/COD quotient = 0 indicating that Acid Orange 154 is not rapidly biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT 20010/C
Batch-Nr: 149
Purity ca: 70 %
Appearance: solid
Solubility: >1 g/I miscible [in water]
Storage: room temperature
Expiration date: 12/99

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

2 h

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 148 ± 3 °C
- Sample weight: 5 mg of the test substance
- Estimation Technique: Titration (potentiometric) METROHM Titroprozessor 670.

Reference substance:

other: Potassium hydrogen phthalate solution (Merck-Nr. 11769)

Remarks on result:

not measured/tested

Parameter:

COD

Value:

1 100 mg O2/g test mat.

Results with reference substance:

The COD of Potassium hydrogen phthalate is 202 mg O2/l (Validity: 200 ± 8 mg O2/l)

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The COD of FAT 20010/C was found to be 1100 mg 02/g

Executive summary:

A predetermined amount of the test substance FAT 20010/C dissolved in water is oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate is determined by titration with standardized ferrous ammonium sulphate. The test was carried out according to DIN 38409 - H 41-1 (1980) corresponding to EEC Directive 92 / 69 / C.6. (1992). The test substance was tested with a sample weight of 5.0 mg. The reference substance was Potassium hydrogen phthalate. It was tested with 20 ml of a 0.17 g/l solution. Based on the findings of the study, the COD of FAT 20010/C was found to be 1100 mg O2/g. The COD of the reference substance is: 202 mg O2/l. In another experiment, the BOD of FAT 20010/C was found to be 0 mg O2/g. The BOD/COD quotient was zero indicating no biodegradation was observed.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT 20010/C
Batch-Nr: 149
Purity ca: 70 %
Appearance: solid
Solubility: >1 g/I miscible [in water]
Storage: room temperature
Expiration date: 12/99

Oxygen conditions:

aerobic

Inoculum or test system:

other: Activated sludge of a communal sewage treatment plant Ara Reinach collected on 09.05.95

Duration of test (contact time):

28 d

Initial conc.:

150 mg/L

Based on:

DOC

Details on study design:

TEST CONDITIONS
- Test temperature: 20 - 25 °C (room temperature)
- Lighting: indirect daylight

Reference substance:

diethylene glycol

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

Adsorption: The adsorption of the test substance FAT 20010/C after 3 hours was: 0 %
Total Elimination: (regarding the adsorption and the biodegradation) The total elimination of the test substance FAT 20010/C after 28 days was: 0 %

Results with reference substance:

The biodegradation, corrected by the blank control and measured as DOC (mg/I) is: 98 % on 7th day (average of 2 tests running in parallel).

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The biodegradation of the test substance FAT 20010/C after 28 days was 0 %

Executive summary:

The inherent biodegradability of FAT 20010/C was determined in a Zahn-Wellens / EMPA ­Test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1992. The test substance FAT 20010/C and the reference substance Diethylenglycol, respectively, were tested in concentrations of 150 mg/I DOC.

Adsorption:

The adsorption of the test substance FAT 20010/C after 3 hours was: 0 %

Biodegradation:

The biodegradation of the test substance FAT 20010/C after 28 days was: 0 %

Total Elimination: (regarding the adsorption and the biodegradation) The total elimination of the test substance FAT 20010/C after 28 days was: 0 % (average of 2 tests running in parallel)

Description of key information

The test substance FAT 20010 can be regarded as not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Additional information

The inherent biodegradability of the test substance FAT 20010/C was determined in a GLP-compliant study in Zahn-Wellens / EMPA Test according to OECD Guideline 302B. The test substance FAT 20010/C and the reference substance Diethylene glycol, respectively, were tested in concentrations of 150 mg/I DOC. The biodegradation of the test substance FAT 20010/C after 28 days was 0 %. Individual BOD5 and COD estimation were done in GLP-compliant studies according to ISO 5815 and EEC 92/69/C.6 (1992), respectively. The biochemical oxygen demand of FAT 20010/C was determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of micro-organisms. The biochemical oxygen demand of FAT 20010/C was determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations diluted in water saturated with dissolved oxygen that contains a seed of micro-organisms. The BOD5 and COD of FAT 20010/C was found to be 0 mg O2/g and 1100 mg O2/g, respectively.

Few non-GLP compliant studies performed in the 70’s have reported the following BOD5 and COD values for FAT 20010:

BOD5 = 52 mg/g O2

COD = 554 mg/g O2

BOD5 = 0 mg/g O2

Relevant is the BOD5/COD quotient which is not >0.5, therefore the substance is not indicative of rapid degradation. Taking into consideration the data from key study and supporting studies, the test substance FAT 20010 can be regarded as not biodegradable..