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EC number: 213-254-4 | CAS number: 932-64-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Remarks:
- in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study run to a detailed method. Not GLP.
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Ten female guinea pigs were used in the treatment group. The compound was administered in a series of ten "sensitizing" injections into the lower back and flanks of the guinea pigs. Two weeks after administration of the tenth sensitizing injection, a challenge injection of 0.05 mL was administered. The volume of the first injection was 0.05 mL and that of the other nine was 0.10 mL.
- GLP compliance:
- not specified
- Type of study:
- other: K. Landsteiner and J. Jacobs, "Studies on the Sensitization of Animals with Simple Chemical Compounds," J. Exp. Med. 61, 643-656 (1935).
- Justification for non-LLNA method:
- Study conducted in 1985 prior to adoption of the LLNA test as the default testing method.
- Specific details on test material used for the study:
- Batch No.: not specified
Purity: not specified - Species:
- guinea pig
- Strain:
- not specified
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 363-438 g
- Housing: The animals were housed individually.
- Diet (e.g. ad libitum): The animals were fed commercial laboratory stock diet ad libitum supplemented by daily lettuce, cabbage, or apples because of their need for dietary fiber.
No additional data - Route:
- intradermal
- Vehicle:
- corn oil
- Concentration / amount:
- 0.1%
- Route:
- intradermal
- Vehicle:
- corn oil
- Concentration / amount:
- 0.1%
- No. of animals per dose:
- 10
- Details on study design:
- MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 10
- Test groups: ten female animals
- Site: lower back and flanks
- Duration: 24 hours
- Concentrations: The volume of the first injection was 0.05 mL and that of the other nine was 0.10 mL .
B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: Two weeks after the topical induction.
- Exposure period: 24 hours
- Test groups: ten female animals
- Site: lower back and flanks
- Concentrations: 0.05 mL
No additional data - Challenge controls:
- None stated
- Positive control substance(s):
- not specified
- Key result
- Group:
- test chemical
- Dose level:
- 0.1%
- Total no. in group:
- 10
- Remarks on result:
- other: Review of the data collected for each guinea pig in the treatment group indicates that all challenge injections were within the limits of the reactions recorded during the sensitizing period.
- Remarks:
- The guinea pig skin sensitization study did not show NTO to be a sensitizer.
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The material did not induce sensitization in the intradermal guinea pig assay.
Reference
Review of the data collected for each guinea pig in the treatment group indicates that all challenge injections were within the limits of the reactions recorded during the sensitizing period. The guinea pig skin sensitization study did not show the test substance to be a sensitizer.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
An intradermal guinea pig assay on NTO was carried out (London and Smith, 1985). Ten female guinea pigs were treated with concentrations of 0.1% NTO in corn oil. The compound was administered in a series of ten sensitizing injections into the lower back and flanks of the guinea pigs. Injections were made randomly over the test area. Twenty four hours after injection the reaction was scored for erythema and swelling. Two weeks after administration of the tenth sensitizing injection, the lower back and flanks of each guinea pig were clipped free of hair and a challenge injection of 0.05 mL was administered. The reaction of each animal was graded 24 hr later and compared with those from the sensitising injections. All challenge injections were within the limits of the reactions recorded during the sensitizing period. Therefore NTO was not shown to be a skin sensitizer.
Migrated from Short description of key information:
The guinea pig skin sensitisation study did not show NTO to be a skin sensitiser.
Justification for selection of skin sensitisation endpoint:
The study was run to a detailed, scientific method though not to GLP.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
NTO is not classified as a skin sensitizer as the skin sensitization study provided a negative result.
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