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EC number: 213-254-4
CAS number: 932-64-9
NTO: Acute toxicity to fish (Oncorynchus mykiss), 96 hours: EC50>100 mg/L
Photo-induced degradation products: Acute toxicity to Ceriodaphniadubia.
48 hours: LC50 - 14.5 mg/L (used for environmental risk assessment).
Based on ECOSAR v.1 (EPISUITE, 2009), the test substance, NTO, was
predicted to have LC50 of 1760.072 mg/L after 96 hours exposure to fish.
Based on the same computer program EPISUITE, the test substance, NTO,
was predicted to have LC50 of 222.878 mg/L after 48 hours exposure to
The found LC50 value of 800 mg/L with Ceriodaphnia dubia by Kennedy et
al correspond to that value, whereas a study by BAE systems did not
allow to conclude on a definite LC50., what however has been calculated
to be above 9.5 mg/L. In this study it weas found the pH would shift
towards unphysiogically values. Therefore just the NOEC could be derived
(2.4 mg/L). The pH depending toxicity fits also well to published data
by Haley Mark V., Kuperman Roman G., Checkai Ronald T. They found, the
48h LC50 to Ceriodaphnia dubia is 460 mg/L for pH-adjusted media and 62
mg/L for non-pH-adjusted media.
According to the published data (Haley Mark V., Kuperman Roman G.,
Checkai Ronald T.: Aquatic Toxicity of 3-Nitro-l,2,4-triazol-5-one ) the
7 day IC50 values for test subtance to Ceriodaphnia dubia was 57 mg/L.
The NOEC and LOEC values for test substance to Ceriodaphnia dubia were
34 and 66 mg/L, respectively.
According to the Biotechnology Research Institute of NRC, 2010, the 96h
EC50 to aquatic algae was found to be 2323.7 mg/L.
The toxicity to aquatic algae was also examined by AnalyCen Ecotox AS.
The72 hours EC50 value for the test substance NTO, Nitrotriazolone was
found to be 113 mg/L.
According to Haley Mark V.,Kuperman Roman G., Checkai Ronald T. (Aquatic
Toxicity of 3-Nitro-l,2,4-triazol-5-one ), 96h NOEC for the test
substance to Scenedesmus capricornutum is 1375 mg/L; 96h LOEC for the
test substance to Scenedesmus capricornutum is 2680 mg/L.
The calculation by EPIWIN was showing an EC50 of 1329.272 mg/L after 96
hours exposure to Green Algae.
Although NTO is harmless to aquatic species, adverse effects from
photo-induced degradates have been reported by Kennedy et al ( Environ
Toxicolo Chem 36, 2017, p2050 -2057) with LC50 14.5 mg/L in a standard
48hr acute toxicity test with Ceriodaphnia dubia. Therefore an
environmental risk assessment has been conducted.
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