Methyl cinnamate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

N/A

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented, available information contributing to assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

XX-1785

IUPAC Name:

XX-1785

Constituent 2

Reference substance name:

Methyl cinnamate

EC Number:

203-093-8

EC Name:

Methyl cinnamate

Cas Number:

103-26-4

Molecular formula:

C10H10O2

IUPAC Name:

methyl 3-phenylacrylate

Test material form:

solid: crystalline

Details on test material:

Chemical name: Methyl cinnamate

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Sprague-Dawley albino rats of both sexes (weighing 150 to 250 grams) were used in the acute oral toxicity studies.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

five (fasted overnight) were given exploratory doses of a test compound by intubation.

Doses:

6 g/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

In order to find proper dosage levels for LD50 determinations, five to six rats (fasted overnight) were given exploratory doses of a test compound by intubation. Following dosing, all animals were kept for a two-day observation period. The toxic signs and mortality were recorded at one, four, 24, and 48 hours. Based on the results obtained from the range-finding study, the determination of the LD50 of each test material was initiated by giving graded dosage levels to six groups of five rats of both sexes via the same route. Where the range-finding study results indicated the test substance to be relatively nontoxic, a single group of five rats of both sexes was dosed at 6 grams or 6 ml/kg. Higher dosages were not used in order to conserve material and LD50's would have little meaning. Toxic signs and mortality were recorded at one and four hours and then once daily for a period of 14 days. A necropsy was performed on all animals that died during the study and on those killed by cervical dislocation at termination.

Statistics:

N/A

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 610 mg/kg bw

Based on:

test mat.

95% CL:

> 2 000 - < 3 410

Remarks on result:

other: Clinical signs of toxicity were depression and decrease in respiration at 3160 mg/kg and higher.

Mortality:

no details known, as certain sections of the report were blanked out for confidentiality reasons by the data owner.

Clinical signs:

other: Depression, decrease in respiration at 3.16 g/kg and higher

Gross pathology:

The stomachs of animals that died 24 hours following the administration of test article were filled with fluid. No tissue damage was seen in any animal killed at the termination of the study.

Other findings:

N/A

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Based on the results in this acute study, LD50 (oral, rat) is considered to be 2610 mg/kg body weight.

Executive summary:

This study was performed to evaluate the acute toxicity of various test substances, amongst them methyl cinnamate (the other substance identities were blanket out by data owner for confidentiality reasons). Rats were applied at dosage levels up to 6 g/kg body weight. five to six rats (fasted overnight) were given exploratory doses of a test compound by intubation. Following dosing, all animals were kept for a two-day observation period. Depression and decrease in respiration were observed at 3.16 g/kg and higher. The stomachs of animals that.died 24 hours following the administration of test article were filled with fluid. No tissue damage was seen in any animal killed at the termination of the study.

Therefore, it is concluded that methyl cinnamate is considered non-classifiable according to CLP (Regulation EC No 1272/2008) in this acute oral toxicity study.