Sulfuric acid, C10-12 (even numbered)-alkyl esters, sodium salts

Short-term toxicity to fish

Description of key information

48h LC50 (Cyprinus carpio pre-larvae, mortality) = 13 mg/L (nominal concentration, Japanese Industrial Standard JIS K0102)
96h LC50 (Cyprinodon variegates) = 4.1 mg/L (measured concentration, marine water, ASTM E-35 1980)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

13 mg/L

Marine water fish

Marine water fish

Effect concentration:

4.1 mg/L

Additional information

Since no reliable short-term fish test is available for C10-12AS Na (CAS 85049-86-1), in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5, a read-across to other structurally related category members was conducted. Read-across data from the following substances were used: C10AS Na (CAS 142-87-0) and C12AS Na (CAS 151-21-3), since toxicity of alkyl sulfates is dependent on the carbon chain length.

In the key, non-GLP study performed according to Japanese Industrial Standard JIS K0102, 1-week old carp pre-larvae (Cyprinus carpio) were exposed to C10AS Na for 48 hours under static conditions (Kikuchi et al. 1976a). Based on pre-larvae mortality, the LC50 value was 13 mg/L (nominal concentrations in agreement with the measured ones). Since the obtained value was the most critical, it is considered as a reliable short-term LC50 value for fish. The same authors provide supporting information on Carp egg hatching rate influenced by C10AS Na (Kikuchi 1976b). Fish eggs were exposed for 96 hours to the test concentrations up to 100 mg/L (nominal concentrations in agreement with the measured ones). The study followed Japanese Industrial Standard JIS K0102. Inhibition of hatching rate could not be calculated because hatching rate was higher at 70 mg/L (ca. 40%) than at 50 mg/L (ca. 12%). No hatching at 100 mg/L was observed. Hatching rate in the control group was 87.3%. Another supporting, non-GLP study performed according to ISO 7346-1 guidance is available (Richterich 2001). Mortality of zebrafish (Danio rerio) was determined after 24, 48, 72 and 96 hours of exposure to five test concentrations up to 1000 mg/L of product containing 30% of active ingredient and 70% water (nominal concentrations). Analytical monitoring was not performed. The results showed that the 96-hour LC50 value was 177 mg a.i./L (nominal concentration).

Toxic effects of C12AS Na (CAS 151-21-3) to fathead minnow (Pimephales promelas) were studied in a non-GLP test performed similar to OECD guideline 203 (Rawlings 2004). Fish were exposed in a flow-through system to five test concentrations up to 48 mg/L of nominal concentration, corresponding to 40 mg/L of measured concentration. Mortality of fish was evaluated every 24 hours. The test substance was determined to have an 96-hour LC50 of 29 mg/L (measured concentration). Moreover, the short term effects of C12AS Na (CAS 151-21-3) were studied on the marine sheepshead minnow Cyprinodon variegatus (Roberts et al. 1982). This non-GLP test was performed according to ASTM E-35 (1980) method. Fish were exposed to five test concentrations in brackish water (salinity 10 ‰) under static conditions. The 96h LC50 value of 4.1 mg/L was determined (measured concentration).

Due to structural and property similarities with the tested category members, it can be concluded that the above results apply also to C10-12AS Na (CAS 85049-86-1). The LC50 value to freshwater fish is 13 mg/L and to marine fish 4.1 mg/L.