1,4-dimethoxy-2-nitrobenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed GLP and OECD guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Report date:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dimethoxy-2-nitrobenzene

EC Number:

201-903-4

EC Name:

1,4-dimethoxy-2-nitrobenzene

Cas Number:

89-39-4

Molecular formula:

C8H9NO4

IUPAC Name:

1,4-dimethoxy-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): Nitrohydrochinondimethylether

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

average weight of the animals at the begin of the test: male 192.1 grams female: 181.7 grams
age of the animals at the begin of the test: male: 7 weeks, female: 9 weeks
source of the animals: Hoechst AG breeding colony
Temperature: 22 +-3°C; rel. humidity: 50 +-20% ; light for 12 hours per day
diet: rat standard diet (Altromin 1324) ad libitum
water: tap water ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: sesame oil

Doses:

2000, 2500, 3150 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 220 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

2 120 mg/kg bw

Based on:

test mat.

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

2 330 mg/kg bw

Based on:

test mat.

Mortality:

2000 mg/kg bw male: 2/5, female: 1/5
2500 mg/kg bw male: 4/5, female: 3/5
3150 mg/kg bw male: 5/5, female: 5/5

Clinical signs:

other: Impaired consciousness, impaired locomotor system, reduced reflexes, irregular breathing, tremor, constricted palpebral fissures, bloody incrusted eyelids, margins and snouts. Surviving animals were free of symptoms after five days.

Gross pathology:

In animals found dead pale spleen and liver, enhanced lobular pattern of the liver, blood in the urine and a yellow mucous mass filling the small intestines was observed. Surviving animals were free of macroscopic findings.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: expert judgment

Conclusions:

LD50 male: 2120 mg/kg bw
LD50 female: 2330 mg/kg bw

Executive summary:

Acute toxicity of Nitrohydrochinondimethylether was tested according to OECD 401. Following this protocol doses of 2000, 2500, and 3150 mg/kg bw were administered by gavage to five male and five female rats per dose.

 

During the observation period of 14 days mortality occured at doses of 2000 (male: 2/5, female: 1/5), 2500 (male: 4/5, female: 3/5), and 3150 mg/kg bw (male: 5/5, female: 5/5). LD50 values of 2120 (male) and 2330 mg/kg bw (female) were calculated using probit analysis.

 

Animals showed impaired consciousness, impairment of locomotory system, reduced reflexes, irregular breathing, tremor, constricted palpebral fissures, bloody incrusted eyelids, margins and snouts. Surviving animals were free of symptoms after five days. In animals found dead pale spleen and liver, enhanced lobular pattern of the liver, blood in the urine and a yellow mucous mass filling the small intestines was observed. Surviving animals were free of macroscopic findings. Body weight gain was not impaired.