1,4-dimethoxy-2-nitrobenzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991 - 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study according to guideline. Solvent was used.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dimethoxy-2-nitrobenzene

EC Number:

201-903-4

EC Name:

1,4-dimethoxy-2-nitrobenzene

Cas Number:

89-39-4

Molecular formula:

C8H9NO4

IUPAC Name:

1,4-dimethoxy-2-nitrobenzene

Details on test material:

yellow cristals

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

For a better dispersion of the test substance the emulgator TWEEN 80 was added.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Brachydanio rerio, self breeded by HOECHST AG Kastengrund

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22 +-1°C

pH:

7,7 - 8,5

Dissolved oxygen:

5.1 - 8.7 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

29.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

40.8 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

56.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of the substance under the test conditions was 40.8 mg/L.